PMID- 12423674
OWN - NLM
STAT- MEDLINE
DCOM- 20030624
LR  - 20190726
IS  - 0028-3908 (Print)
IS  - 0028-3908 (Linking)
VI  - 43
IP  - 6
DP  - 2002 Nov
TI  - Nicotinic receptors regulate the release of glycine onto lamina X neurones of the 
      rat spinal cord.
PG  - 1044-54
AB  - Whole-cell patch clamp recordings were performed on neurones in the lamina X of 
      rat spinal cord slices in order to characterize glycinergic synaptic currents and 
      their modulation by nicotinic acetylcholine receptors. In the presence of TTX, 
      bicuculline and kynurenic acid, glycine-induced currents and miniature 
      glycinergic postsynaptic currents (mIPSCs) were recorded. These currents reversed 
      near the chloride ion equilibrium potential and were blocked by strychnine (1 
      microM). A selective nicotinic acetylcholine receptor (nAChR) agonist 
      1,1-dimethyl-4-phenyl-piperazinium (DMPP), increased the frequency of glycinergic 
      mIPSCs without altering significantly their amplitude distributions or their 
      kinetic properties. The effects of DMPP were mimicked by different nAChRs 
      agonists with the following apparent order of potency: ACh > DMPP > nicotine > 
      cytisine. The effect of DMPP on mIPSCs was blocked by both d-tubocurarine and 
      hexamethonium, and was reduced by dihydro-beta-erythroidine and 
      methyllycaconitine (MLA), antagonists of non alpha7- and alpha7-containing 
      nAChRs, respectively. In the absence of TTX, strychnine-sensitive glycinergic 
      electrically evoked postsynaptic currents (eIPSCs) could be recorded. DMPP 
      blocked the appearance of electrically evoked IPSCs while still inducing the 
      appearance of spontaneous glycine IPSCs. These data demonstrate that neurones 
      surrounding the central canal of the spinal cord present a glycinergic synaptic 
      transmission which is modulated by terminal nAChRs.
FAU - Bradaia, A
AU  - Bradaia A
AD  - Laboratoire de Neurophysiologie Cellulaire et Integree, UMR 7519 CNRS ULP, 21 rue 
      R.Descartes, 67084 Strasbourg Cedex, France.
FAU - Trouslard, J
AU  - Trouslard J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuropharmacology
JT  - Neuropharmacology
JID - 0236217
RN  - 0 (Alkaloids)
RN  - 0 (Anesthetics, Local)
RN  - 0 (Azocines)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Glycine Agents)
RN  - 0 (Nicotinic Agonists)
RN  - 0 (Nicotinic Antagonists)
RN  - 0 (Quinolizines)
RN  - 0 (Receptors, Nicotinic)
RN  - 21019-30-7 (methyllycaconitine)
RN  - 23255-54-1 (Dihydro-beta-Erythroidine)
RN  - 3C9PSP36Z2 (Hexamethonium)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - 53S5U404NU (cytisine)
RN  - 54-77-3 (Dimethylphenylpiperazinium Iodide)
RN  - 6OTE87SCCW (6-Cyano-7-nitroquinoxaline-2,3-dione)
RN  - H030S2S85J (Kynurenic Acid)
RN  - H9Y79VD43J (Strychnine)
RN  - TE7660XO1C (Glycine)
RN  - W9YXS298BM (Tubocurarine)
RN  - X8YN71D5WC (Aconitine)
RN  - Y37615DVKC (Bicuculline)
SB  - IM
MH  - 6-Cyano-7-nitroquinoxaline-2,3-dione/pharmacology
MH  - Aconitine/*analogs & derivatives/pharmacology
MH  - Alkaloids/pharmacology
MH  - Anesthetics, Local/pharmacology
MH  - Animals
MH  - Animals, Newborn
MH  - Azocines
MH  - Bicuculline/pharmacology
MH  - Dihydro-beta-Erythroidine/pharmacology
MH  - Dimethylphenylpiperazinium Iodide/pharmacology
MH  - Drug Interactions
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - Glycine/*metabolism
MH  - Glycine Agents/pharmacology
MH  - Hexamethonium/pharmacology
MH  - In Vitro Techniques
MH  - Kynurenic Acid/pharmacology
MH  - *Membrane Potentials/drug effects/physiology
MH  - Neurons/drug effects/*metabolism
MH  - Nicotinic Agonists/pharmacology
MH  - Nicotinic Antagonists/pharmacology
MH  - Patch-Clamp Techniques/methods
MH  - Quinolizines
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Nicotinic/*physiology
MH  - Spinal Cord/*cytology/physiology
MH  - Strychnine/pharmacology
MH  - Tetrodotoxin/pharmacology
MH  - Time Factors
MH  - Tubocurarine/pharmacology
EDAT- 2002/11/09 04:00
MHDA- 2003/06/25 05:00
CRDT- 2002/11/09 04:00
PHST- 2002/11/09 04:00 [pubmed]
PHST- 2003/06/25 05:00 [medline]
PHST- 2002/11/09 04:00 [entrez]
AID - S0028390802001211 [pii]
AID - 10.1016/s0028-3908(02)00121-1 [doi]
PST - ppublish
SO  - Neuropharmacology. 2002 Nov;43(6):1044-54. doi: 10.1016/s0028-3908(02)00121-1.