PMID- 12423687
OWN - NLM
STAT- MEDLINE
DCOM- 20021212
LR  - 20191025
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 30
IP  - 11
DP  - 2002 Nov
TI  - Radiation pneumonitis in mice: a severe injury model for pneumocyte engraftment 
      from bone marrow.
PG  - 1333-8
AB  - OBJECTIVE: To better understand the process by which pneumocytes can be derived 
      from bone marrow cells, we investigated the in vivo kinetics of such engraftment 
      following lethal irradiation. METHODS: A cohort of lethally irradiated B6D2F1 
      female mice received whole bone marrow transplants (BMT) from age-matched male 
      donors and were sacrificed at days 1, 3, 5, and 7 and months 2, 4, and 6 post-BMT 
      (n = 3 for each time point). Additionally, 2 female mice who had received 200 
      male fluorescence-activated cell sorter (FACS)-sorted CD34(+)lin(-) cells were 
      sacrificed 8 months post-BMT. RESULTS: Lethal irradiation caused histologic 
      evidence of pneumonitis including alveolar breakdown and hemorrhage beginning at 
      day 3. To identify male-derived pneumocytes, simultaneous fluorescence in situ 
      hybridization (FISH) for Y-chromosome and surfactant B messenger RNA was 
      performed on lung tissue. Y(+) type II pneumocytes were engrafted as early as day 
      5 posttransplant, and eventually from 2 to 14% of the pneumocytes were donor 
      derived in individual mice. Co-staining for epithelial-specific cytokeratins 
      demonstrated that by 2 months, marrow-derived pneumocytes could comprise entire 
      alveoli, suggesting that type I cells derived from type II pneumocytes. 
      CONCLUSIONS: We conclude that alveolar lining cells derive from bone marrow cells 
      immediately after acute injury. Also, the CD34(+)lin(-) subpopulation is capable 
      of such pulmonary engraftment.
FAU - Theise, Neil D
AU  - Theise ND
AD  - Department of Pathology, New York University School of Medicine, New York, NY 
      10016, USA. Neil.Theise@med.nyu.edu
FAU - Henegariu, Octavian
AU  - Henegariu O
FAU - Grove, Joanna
AU  - Grove J
FAU - Jagirdar, Jayishree
AU  - Jagirdar J
FAU - Kao, Peter N
AU  - Kao PN
FAU - Crawford, James M
AU  - Crawford JM
FAU - Badve, Sunil
AU  - Badve S
FAU - Saxena, Romil
AU  - Saxena R
FAU - Krause, Diane S
AU  - Krause DS
LA  - eng
GR  - DK53037/DK/NIDDK NIH HHS/United States
GR  - HK69207/HK/PHITPO CDC HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - 0 (Biomarkers)
RN  - 0 (Pulmonary Surfactant-Associated Protein B)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - *Bone Marrow Transplantation
MH  - Cell Differentiation
MH  - *Cell Lineage
MH  - Epithelial Cells/cytology/metabolism
MH  - Female
MH  - Graft Survival
MH  - In Situ Hybridization, Fluorescence
MH  - Lung/*pathology/radiation effects
MH  - Male
MH  - Mice
MH  - Models, Animal
MH  - Pulmonary Alveoli/cytology
MH  - Pulmonary Surfactant-Associated Protein B/genetics
MH  - RNA, Messenger/analysis
MH  - Radiation Chimera
MH  - Radiation Pneumonitis/*therapy
MH  - *Stem Cell Transplantation
MH  - Stem Cells/cytology/metabolism
MH  - Y Chromosome
EDAT- 2002/11/09 04:00
MHDA- 2002/12/13 04:00
CRDT- 2002/11/09 04:00
PHST- 2002/11/09 04:00 [pubmed]
PHST- 2002/12/13 04:00 [medline]
PHST- 2002/11/09 04:00 [entrez]
AID - S0301472X02009311 [pii]
AID - 10.1016/s0301-472x(02)00931-1 [doi]
PST - ppublish
SO  - Exp Hematol. 2002 Nov;30(11):1333-8. doi: 10.1016/s0301-472x(02)00931-1.