PMID- 12424788
OWN - NLM
STAT- MEDLINE
DCOM- 20030116
LR  - 20131121
IS  - 1059-910X (Print)
IS  - 1059-910X (Linking)
VI  - 59
IP  - 4
DP  - 2002 Nov 15
TI  - Effect of time of restriction on the decrease in mitochondrial H2O2 production 
      and oxidative DNA damage in the heart of food-restricted rats.
PG  - 273-7
AB  - In the present study, the question if medium-term (4 months) caloric restriction 
      (40%) decreases mitochondrial H2O2 production and oxidative DNA damage was 
      investigated. Caloric restriction (CR) is the only experimental manipulation that 
      increases maximum life span. Previous long-term CR studies have showed that CR 
      decreases the mitochondrial rate of free radical production in diverse tissues 
      and species. Those studies agree with the idea that the superior longevity of the 
      restricted animals can be partly due to their lower mitochondrial rate of free 
      radical generation. However, caloric restriction effects strongly depend on 
      implementation time. Previous studies have shown that the decrease induced by CR 
      on oxygen radical generation and oxidative damage to mitochondrial DNA occurs 
      after 1 year but not after 6 weeks of restriction. In the present investigation, 
      mitochondrial H2O2 production did not change in medium-term (4 months) caloric 
      restricted animals, and, in agreement with that, no differences were found in 
      either mitochondrial or nuclear oxidative DNA damage between restricted and ad 
      libitum-fed animals. These results confirm the importance of the time of CR 
      implementation, and show that time longer than 4 months is needed to decrease the 
      mitochondrial rate of free radical generation and the oxidative damage to mtDNA 
      in the rat heart.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Gredilla, R
AU  - Gredilla R
AD  - Department of Animal Biology-II, Faculty of Biology, Complutense University, 
      Madrid 28040, Spain.
FAU - Lopez-Torres, M
AU  - Lopez-Torres M
FAU - Barja, G
AU  - Barja G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Microsc Res Tech
JT  - Microscopy research and technique
JID - 9203012
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Animals
MH  - *Caloric Restriction
MH  - *DNA Damage
MH  - Hydrogen Peroxide/*metabolism
MH  - Male
MH  - Mitochondria, Heart/*metabolism
MH  - Oxidation-Reduction
MH  - Oxygen Consumption
MH  - Rats
MH  - Rats, Wistar
MH  - Time Factors
EDAT- 2002/11/09 04:00
MHDA- 2003/01/17 04:00
CRDT- 2002/11/09 04:00
PHST- 2002/11/09 04:00 [pubmed]
PHST- 2003/01/17 04:00 [medline]
PHST- 2002/11/09 04:00 [entrez]
AID - 10.1002/jemt.10204 [doi]
PST - ppublish
SO  - Microsc Res Tech. 2002 Nov 15;59(4):273-7. doi: 10.1002/jemt.10204.