PMID- 12425815
OWN - NLM
STAT- MEDLINE
DCOM- 20030113
LR  - 20061115
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 82
IP  - 16
DP  - 2002 Aug 25
TI  - [Analysis of chromosomal aneuploidy in bronchoscopically gained cells].
PG  - 1090-2
AB  - OBJECTIVE: To study the feasibility of interphase fluorescence in situ 
      hybridization (FISH) in detection of chromosomal aneuploidy in bronchoscopically 
      gained cells from lung cancer patients and early diagnosis of lung cancer. 
      METHOD: Cells gained from endobronchial brushings of 14 patients with lung cancer 
      were examined by FISH with chromosomes 7, 8, 9 and 12 specific centromere probes. 
      RESULT: Aneuploidy, mainly featured in gains of chromosomes 7, 8 and 12 and loss 
      of chromosome 9 were observed in all of the 7 cases with cells from endobronchial 
      brushings diagnosed as or suspicious of cancer cells by cytology. Aneuploidies of 
      chromosomes 7, 8, and 9 were found in 3 of other 7 cases that appeared to be 
      normal by cytologic criteria. CONCLUSION: Interphase FISH is feasible in 
      detecting aneuploidy associated with malignancy in bronchoscopically gained cells 
      that do not meet the criteria of malignancy by conventional cytologic study. This 
      method may be applied to early diagnosis of lung cancer.
FAU - Gao, Shugeng
AU  - Gao S
AD  - Department of Thoracic Surgery, Cancer Hospital, Chinese Academy of Medical 
      Sciences and Peking Union Medical University, Beijing 100021, China.
FAU - Dong, Xiangyang
AU  - Dong X
FAU - Cheng, Shujun
AU  - Cheng S
FAU - Wang, Liangjun
AU  - Wang L
FAU - Zhang, Dechao
AU  - Zhang D
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
SB  - IM
MH  - *Aneuploidy
MH  - Bronchoscopy
MH  - Chromosomes, Human, Pair 12/genetics
MH  - Chromosomes, Human, Pair 7/genetics
MH  - Chromosomes, Human, Pair 8/genetics
MH  - Chromosomes, Human, Pair 9/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/*genetics/pathology
EDAT- 2002/11/12 04:00
MHDA- 2003/01/14 04:00
CRDT- 2002/11/12 04:00
PHST- 2002/11/12 04:00 [pubmed]
PHST- 2003/01/14 04:00 [medline]
PHST- 2002/11/12 04:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2002 Aug 25;82(16):1090-2.