PMID- 12426563
OWN - NLM
STAT- MEDLINE
DCOM- 20021219
LR  - 20220330
IS  - 1529-2908 (Print)
IS  - 1529-2908 (Linking)
VI  - 3
IP  - 12
DP  - 2002 Dec
TI  - Indirect activation of naive CD4+ T cells by dendritic cell-derived exosomes.
PG  - 1156-62
AB  - Dendritic cells (DCs) secrete vesicles of endosomal origin, called exosomes, that 
      bear major histocompatibility complex (MHC) and T cell costimulatory molecules. 
      Here, we found that injection of antigen- or peptide-bearing exosomes induced 
      antigen-specific naive CD4+ T cell activation in vivo. In vitro, exosomes did not 
      induce antigen-dependent T cell stimulation unless mature CD8alpha- DCs were also 
      present in the cultures. These mature DCs could be MHC class II-negative, but had 
      to bear CD80 and CD86. Therefore, in addition to carrying antigen, exosomes 
      promote the exchange of functional peptide-MHC complexes between DCs. Such a 
      mechanism may increase the number of DCs bearing a particular peptide, thus 
      amplifying the initiation of primary adaptive immune responses.
FAU - Thery, Clotilde
AU  - Thery C
AD  - INSERM U520, Institut Curie, 12 rue Lhomond, 75005 Paris, France.
FAU - Duban, Livine
AU  - Duban L
FAU - Segura, Elodie
AU  - Segura E
FAU - Veron, Philippe
AU  - Veron P
FAU - Lantz, Olivier
AU  - Lantz O
FAU - Amigorena, Sebastian
AU  - Amigorena S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20021111
PL  - United States
TA  - Nat Immunol
JT  - Nature immunology
JID - 100941354
RN  - 0 (Antigens, CD)
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (Cd86 protein, mouse)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Membrane Glycoproteins)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Antigens, CD/immunology
MH  - B7-1 Antigen/immunology
MH  - B7-2 Antigen
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - Cell Communication/immunology
MH  - Dendritic Cells/*immunology/ultrastructure
MH  - Flow Cytometry
MH  - Histocompatibility Antigens Class II/immunology
MH  - *Lymphocyte Activation/immunology
MH  - Membrane Glycoproteins/immunology
MH  - Mice
MH  - Secretory Vesicles/immunology
EDAT- 2002/11/12 04:00
MHDA- 2002/12/20 04:00
CRDT- 2002/11/12 04:00
PHST- 2002/07/01 00:00 [received]
PHST- 2002/10/01 00:00 [accepted]
PHST- 2002/11/12 04:00 [pubmed]
PHST- 2002/12/20 04:00 [medline]
PHST- 2002/11/12 04:00 [entrez]
AID - ni854 [pii]
AID - 10.1038/ni854 [doi]
PST - ppublish
SO  - Nat Immunol. 2002 Dec;3(12):1156-62. doi: 10.1038/ni854. Epub 2002 Nov 11.