PMID- 12426764
OWN - NLM
STAT- MEDLINE
DCOM- 20030213
LR  - 20231213
IS  - 0016-254X (Print)
IS  - 0016-254X (Linking)
VI  - 93
IP  - 9
DP  - 2002 Sep
TI  - Molecular cloning of two guinea pig liver gap junction proteins, connexin32 and 
      connexin26.
PG  - 178-88
AB  - Intercellular coupling of hepatocytes through gap junctions facilitates exchange 
      of small metabolites or ions, and contributes to maintenance of tissue 
      homeostasis. As protein constituents of the liver gap junction channels, 
      connexin32 (Cx32) and Cx26 have been identified. By use of rat cDNA probes, we 
      cloned cDNAs for guinea pig homologs of Cx32 and Cx26, and compared their amino 
      acid sequences with those of other species. The deduced primary structure of 
      guinea pig Cx32 was 283 amino acids long and contained 98% identical amino acids 
      to the rat and human Cx32. Only six amino acid exchanges were detected between 
      the guinea pig and rat Cx32. On the contrary, the deduced amino acid sequence of 
      guinea pig Cx26 (226 amino acids long) was 91 and 89% identical to the rat and 
      human Cx26, respectively. Twenty-one amino acid exchanges were found between the 
      guinea pig and rat, and the divergence was mostly located in cytoplasmic domains 
      of Cx26. These results suggest that Cx26 shows structural diversity between 
      species, while Cx32 is highly conserved.
FAU - Kuraoka, Akio
AU  - Kuraoka A
AD  - Departments of Anatomy and Cell Biology, Graduate School of Medical Sciences, 
      Kyushu University, Fukuoka 812-8582, Japan. qura@anat1.med.kyushu-u.ac.jp
FAU - Nakashima, Torahiko
AU  - Nakashima T
FAU - Kawabuchi, Masaru
AU  - Kawabuchi M
FAU - Shibata, Yosaburo
AU  - Shibata Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Japan
TA  - Fukuoka Igaku Zasshi
JT  - Fukuoka igaku zasshi = Hukuoka acta medica
JID - 9423321
RN  - 0 (Connexins)
RN  - 0 (GJB2 protein, human)
RN  - 0 (Gjb2 protein, rat)
RN  - 127120-53-0 (Connexin 26)
SB  - IM
MH  - Animals
MH  - Cloning, Molecular
MH  - Connexin 26
MH  - Connexins/*genetics
MH  - Female
MH  - Guinea Pigs
MH  - Humans
MH  - Liver/*chemistry
MH  - Rats
MH  - Sequence Analysis, Protein
MH  - Gap Junction beta-1 Protein
EDAT- 2002/11/13 04:00
MHDA- 2003/02/14 04:00
CRDT- 2002/11/13 04:00
PHST- 2002/11/13 04:00 [pubmed]
PHST- 2003/02/14 04:00 [medline]
PHST- 2002/11/13 04:00 [entrez]
PST - ppublish
SO  - Fukuoka Igaku Zasshi. 2002 Sep;93(9):178-88.