PMID- 12427830
OWN - NLM
STAT- MEDLINE
DCOM- 20021118
LR  - 20200225
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 22
IP  - 22
DP  - 2002 Nov 15
TI  - Increased expression of brain-derived neurotrophic factor induces formation of 
      basal dendrites and axonal branching in dentate granule cells in hippocampal 
      explant cultures.
PG  - 9754-63
AB  - During limbic epileptogenesis in vivo the dentate granule cells (DGCs) exhibit 
      increased expression of brain-derived neurotrophic factor (BDNF), followed by 
      striking morphologic plasticities, namely the formation of basal dendrites and 
      the sprouting of mossy fibers. We hypothesized that increased expression of BDNF 
      intrinsic to DGCs is sufficient to induce these plasticities. To test this 
      hypothesis, we transfected DGCs in rat hippocampal slice cultures with BDNF or 
      nerve growth factor (NGF) via particle-mediated gene transfer, and we visualized 
      the neuronal processes with cotransfected green fluorescent protein. Transfection 
      with BDNF produced significant increases in axonal branch and basal dendrite 
      number relative to NGF or empty vector controls. Structural changes were 
      prevented by the tyrosine kinase inhibitor K252a. Thus increased expression of 
      BDNF within DGCs is sufficient to induce these morphological plasticities, which 
      may represent one mechanism by which BDNF promotes limbic epileptogenesis.
FAU - Danzer, Steve C
AU  - Danzer SC
AD  - Department of Medicine (Neurology), Duke University Medical Center, Durham, North 
      Carolina 27710, USA.
FAU - Crooks, Kristy R C
AU  - Crooks KR
FAU - Lo, Donald C
AU  - Lo DC
FAU - McNamara, James O
AU  - McNamara JO
LA  - eng
GR  - T32 NS007370/NS/NINDS NIH HHS/United States
GR  - NS17771/NS/NINDS NIH HHS/United States
GR  - NS32334/NS/NINDS NIH HHS/United States
GR  - P01 NS017771/NS/NINDS NIH HHS/United States
GR  - NS07370/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Axons/drug effects/*physiology
MH  - Biolistics
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics/pharmacology
MH  - Cell Survival/drug effects/physiology
MH  - Cells, Cultured
MH  - Dendrites/drug effects/*physiology
MH  - Dentate Gyrus/cytology/*metabolism
MH  - Epilepsy/etiology
MH  - Hippocampus/cytology
MH  - Immunohistochemistry
MH  - In Vitro Techniques
MH  - Male
MH  - Neurons/cytology/drug effects/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC6757848
EDAT- 2002/11/13 04:00
MHDA- 2002/11/26 04:00
PMCR- 2003/05/15
CRDT- 2002/11/13 04:00
PHST- 2002/11/13 04:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/11/13 04:00 [entrez]
PHST- 2003/05/15 00:00 [pmc-release]
AID - 22/22/9754 [pii]
AID - 6995 [pii]
AID - 10.1523/JNEUROSCI.22-22-09754.2002 [doi]
PST - ppublish
SO  - J Neurosci. 2002 Nov 15;22(22):9754-63. doi: 10.1523/JNEUROSCI.22-22-09754.2002.