PMID- 12427927
OWN - NLM
STAT- MEDLINE
DCOM- 20030124
LR  - 20200826
IS  - 1350-0872 (Print)
IS  - 1350-0872 (Linking)
VI  - 148
IP  - Pt 11
DP  - 2002 Nov
TI  - Glycogen-accumulating organisms in laboratory-scale and full-scale wastewater 
      treatment processes.
PG  - 3353-3364
LID - 10.1099/00221287-148-11-3353 [doi]
AB  - Laboratory-scale sequencing batch reactors (SBRs) as models for wastewater 
      treatment processes were used to identify glycogen-accumulating organisms (GAOs), 
      which are thought to be responsible for the deterioration of enhanced biological 
      phosphorus removal (EBPR). The SBRs (called Q and T), operated under alternating 
      anaerobic-aerobic conditions typical for EBPR, generated mixed microbial 
      communities (sludges) demonstrating the GAO phenotype. Intracellular glycogen and 
      poly-beta-hydroxyalkanoate (PHA) transformations typical of efficient EBPR 
      occurred but polyphosphate was not bioaccumulated and the sludges contained 1.8% 
      P (sludge Q) and 1.5% P (sludge T). 16S rDNA clone libraries were prepared from 
      DNA extracted from the Q and T sludges. Clone inserts were grouped into 
      operational taxonomic units (OTUs) by restriction fragment length polymorphism 
      banding profiles. OTU representatives were sequenced and phylogenetically 
      analysed. The Q sludge library comprised four OTUs and all six determined 
      sequences were 99.7% identical, forming a cluster in the gamma-Proteobacteria 
      radiation. The T sludge library comprised eight OTUs and the majority of clones 
      were Acidobacteria subphylum 4 (49% of the library) and candidate phylum OP10 
      (39% of the library). One OTU (two clones, of which one was sequenced) was in the 
      gamma-Proteobacteria radiation with 95% sequence identity to the Q sludge clones. 
      Oligonucleotide probes (called GAOQ431 and GAOQ989) were designed from the 
      gamma-Proteobacteria clone sequences for use in fluorescence in situ 
      hybridization (FISH); 92% of the Q sludge bacteria and 28% of the T sludge 
      bacteria bound these probes in FISH. FISH and post-FISH chemical staining for PHA 
      were used to determine that bacteria from a novel gamma-Proteobacteria cluster 
      were phenotypically GAOs in one laboratory-scale SBR and two full-scale 
      wastewater treatment plants. It is suggested that the GAOs from the novel cluster 
      in the gamma-Proteobacteria radiation be named 'Candidatus Competibacter 
      phosphatis'.
FAU - Crocetti, Gregory R
AU  - Crocetti GR
AD  - Advanced Wastewater Management Centre, The University of Queensland, St Lucia 
      4072, Australia1.
FAU - Banfield, Jillian F
AU  - Banfield JF
AD  - Department of Geology and Geophysics, University of Wisconsin-Madison, Madison, 
      WI 53706, USA2.
FAU - Keller, Jurg
AU  - Keller J
AD  - Advanced Wastewater Management Centre, The University of Queensland, St Lucia 
      4072, Australia1.
FAU - Bond, Philip L
AU  - Bond PL
AD  - School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK3.
FAU - Blackall, Linda L
AU  - Blackall LL
AD  - Advanced Wastewater Management Centre, The University of Queensland, St Lucia 
      4072, Australia1.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Microbiology (Reading)
JT  - Microbiology (Reading, England)
JID - 9430468
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 9005-79-2 (Glycogen)
SB  - IM
MH  - Bacteria/genetics/*metabolism
MH  - Glycogen/*metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Phenotype
MH  - RNA, Ribosomal, 16S/analysis
MH  - Sewage/*microbiology
MH  - Staining and Labeling
MH  - Waste Disposal, Fluid
MH  - *Waste Management
MH  - Water Purification
EDAT- 2002/11/13 04:00
MHDA- 2003/01/25 04:00
CRDT- 2002/11/13 04:00
PHST- 2002/11/13 04:00 [pubmed]
PHST- 2003/01/25 04:00 [medline]
PHST- 2002/11/13 04:00 [entrez]
AID - 10.1099/00221287-148-11-3353 [doi]
PST - ppublish
SO  - Microbiology (Reading). 2002 Nov;148(Pt 11):3353-3364. doi: 
      10.1099/00221287-148-11-3353.