PMID- 12428794
OWN - NLM
STAT- MEDLINE
DCOM- 20021126
LR  - 20220330
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 118
IP  - 5
DP  - 2002 Nov
TI  - Analysis of the molecular quality of human tissues: an experience from the 
      Cooperative Human Tissue Network.
PG  - 733-41
AB  - The scientific usefulness of the data obtained from tissue analysis is related to 
      specimen quality, which may be affected by conditions that may contribute to the 
      degradation of the specimen before processing and analysis. We determined the 
      usability of nucleic acids extracted from banked human tissues for further 
      molecular analyses. We assayed 151 tissue specimens, storedfor various times at 4 
      divisions of the Cooperative Human Tissue Network, National Cancer Institute, 
      Bethesda, MD, for DNA and RNA degradation. Simple electrophoresis, polymerase 
      chain reaction (PCR), reverse-transcriptase (RT)-PCR, and Northern blot analysis 
      were compared to determine the optimal quality control procedure. In addition, a 
      time course degradation procedure was performed on human lung tissue. Gel 
      electrophoresis was as informative as PCR, RT-PCR, and Northern blot analysis in 
      determining the molecular usefulness of the human tissues. Overall, 80% of the 
      stored human tissues had good-quality DNA, and 60% had good-quality RNA. 
      Electrophoresis procedures for DNA and RNA offer a quick and valuable measure of 
      the molecular quality of stored human tissues. The DNA and RNA degradation of one 
      tissue type (lung) was stable for both nucleic acids for up to 5 hours after 
      excision.
FAU - Jewell, Scott D
AU  - Jewell SD
AD  - Department of Pathology, Ohio State University, Columbus 43210, USA.
FAU - Srinivasan, Mythily
AU  - Srinivasan M
FAU - McCart, Linda M
AU  - McCart LM
FAU - Williams, Nita
AU  - Williams N
FAU - Grizzle, William H
AU  - Grizzle WH
FAU - LiVolsi, Virginia
AU  - LiVolsi V
FAU - MacLennan, Greg
AU  - MacLennan G
FAU - Sedmak, Daniel D
AU  - Sedmak DD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Blotting, Northern
MH  - DNA/*analysis
MH  - Electrophoresis
MH  - Humans
MH  - National Institutes of Health (U.S.)
MH  - RNA/*analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Specimen Handling/*methods
MH  - Time Factors
MH  - *Tissue Banks
MH  - United States
EDAT- 2002/11/14 04:00
MHDA- 2002/11/28 04:00
CRDT- 2002/11/14 04:00
PHST- 2002/11/14 04:00 [pubmed]
PHST- 2002/11/28 04:00 [medline]
PHST- 2002/11/14 04:00 [entrez]
AID - 10.1309/VPQL-RT21-X7YH-XDXK [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2002 Nov;118(5):733-41. doi: 10.1309/VPQL-RT21-X7YH-XDXK.