PMID- 12429120
OWN - NLM
STAT- MEDLINE
DCOM- 20030225
LR  - 20191025
IS  - 0965-1748 (Print)
IS  - 0965-1748 (Linking)
VI  - 32
IP  - 12
DP  - 2002 Dec
TI  - A digestive beta-glucosidase from the salivary glands of the termite, Neotermes 
      koshunensis (Shiraki): distribution, characterization and isolation of its 
      precursor cDNA by 5'- and 3'-RACE amplifications with degenerate primers.
PG  - 1681-9
AB  - beta-Glucosidase activity [EC 3.2.1.21] was measured in the salivary glands and 
      the gut of wood-eating termite, Neotermes koshunensis (Shiraki). 75% of the 
      activity was detected in the salivary glands, whereas 15% of the activity was 
      present in the hindgut, where numerous symbiotic flagellates reside. The salivary 
      beta-glucosidase was partially purified by ion-exchange and gel filtration 
      chromatography. The molecular weight of the salivary beta-glucosidase was 60 kDa, 
      and the K(m) value on cellobiose was 2.5 mM. Its optimal pH was 5.6 and the 
      activity was stable from 20 degrees C up to 45 degrees C. In addition to 
      cellobiose, p-nitrophenyl-beta-D-fucopyranoside and laminaribiose were 
      efficiently hydrolyzed by the salivary beta-glucosidase. Degenerate PCR using 
      primers designed from N-terminal amino acid sequences of the salivary 
      beta-glucosidase resulted in a cDNA fragment of 1730 bp, encoding 498 amino acids 
      and with sequence similarity to glycosyl hydrolase family 1. 
      Reverse-transcription (RT)-PCR showed that this beta-glucosidase is produced only 
      in the salivary glands.
FAU - Tokuda, Gaku
AU  - Tokuda G
AD  - Center of Molecular Biosciences, University of the Ryukyus, Nishihara, Okinawa 
      903-0213, Japan. tokuda@comb.u-ryukyu.ac.jp
FAU - Saito, Hitoshi
AU  - Saito H
FAU - Watanabe, Hirofumi
AU  - Watanabe H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Insect Biochem Mol Biol
JT  - Insect biochemistry and molecular biology
JID - 9207282
RN  - 0 (3' Untranslated Regions)
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - EC 3.2.1.21 (beta-Glucosidase)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - 5' Untranslated Regions/genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - DNA Primers
MH  - DNA, Complementary
MH  - Isoptera/enzymology/*genetics
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Salivary Glands/*enzymology
MH  - Substrate Specificity
MH  - beta-Glucosidase/chemistry/*genetics/metabolism
EDAT- 2002/11/14 04:00
MHDA- 2003/02/26 04:00
CRDT- 2002/11/14 04:00
PHST- 2002/11/14 04:00 [pubmed]
PHST- 2003/02/26 04:00 [medline]
PHST- 2002/11/14 04:00 [entrez]
AID - S096517480200108X [pii]
AID - 10.1016/s0965-1748(02)00108-x [doi]
PST - ppublish
SO  - Insect Biochem Mol Biol. 2002 Dec;32(12):1681-9. doi: 
      10.1016/s0965-1748(02)00108-x.