PMID- 12430903
OWN - NLM
STAT- MEDLINE
DCOM- 20030717
LR  - 20191025
IS  - 0925-5710 (Print)
IS  - 0925-5710 (Linking)
VI  - 76 Suppl 2
DP  - 2002 Aug
TI  - Cytogenetic and FISH studies in myelodysplasia, acute myeloid leukemia, chronic 
      lymphocytic leukemia and lymphoma.
PG  - 65-74
AB  - Conventional cytogenetic studies are widely used today to diagnose and manage 
      patients with hematological malignancies. The application of fluorescence in situ 
      hybridization (FISH) with chromosome-specific DNA probes helps to further define 
      molecular subclasses and cytogenetic risk categories for patients with these 
      disorders. Moreover, FISH permits analysis of proliferating (metaphase cells) and 
      non-proliferating (interphase nuclei) cells, and is useful in establishing the 
      percentage of neoplastic cells before and after therapy (minimal residual 
      disease). For patients with myelodysplasia or acute myeloid leukemia, these 
      chromosome techniques are important for accurate diagnosis and classification of 
      disease and to help decide treatment and monitor response to therapy. 
      Conventional cytogenetic studies have been problematic in chronic lymphocytic 
      leukemia because the neoplastic cells divide infrequently. However, interphase 
      FISH studies now permit detection of chromosome anomalies with prognostic 
      significance in chronic lymphocytic leukemia. The World Health Organization 
      recognizes that genetic anomalies are one of the most reliable criteria for 
      classification of malignant lymphomas. New methods to extract individual nuclei 
      from paraffin-embedded tissue are now available which permit the use of 
      interphase FISH to detect important chromosome anomalies in lymphoma.
FAU - Dewald, Gordon W
AU  - Dewald GW
AD  - Division of Laboratory Genetics, Mayo Clinic, Rochester, MN, USA.
LA  - eng
PT  - Journal Article
PT  - Review
PL  - Japan
TA  - Int J Hematol
JT  - International journal of hematology
JID - 9111627
SB  - IM
MH  - Acute Disease
MH  - Chromosome Aberrations
MH  - Cytogenetic Analysis
MH  - Hematologic Neoplasms/diagnosis/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/diagnosis/genetics
MH  - Leukemia, Myeloid/diagnosis/genetics
MH  - Lymphoma/diagnosis/genetics
MH  - Multiple Myeloma/diagnosis/genetics
RF  - 49
EDAT- 2002/11/15 04:00
MHDA- 2003/07/18 05:00
CRDT- 2002/11/15 04:00
PHST- 2002/11/15 04:00 [pubmed]
PHST- 2003/07/18 05:00 [medline]
PHST- 2002/11/15 04:00 [entrez]
AID - 10.1007/BF03165090 [doi]
PST - ppublish
SO  - Int J Hematol. 2002 Aug;76 Suppl 2:65-74. doi: 10.1007/BF03165090.