PMID- 12431709
OWN - NLM
STAT- MEDLINE
DCOM- 20030131
LR  - 20191106
IS  - 1385-299X (Print)
IS  - 1385-299X (Linking)
VI  - 10
IP  - 2
DP  - 2002 Oct
TI  - Microdialysis in trigeminal ganglia.
PG  - 102-8
AB  - Recent evidence demonstrates that neurons in sensory ganglia contribute to 
      sensory signaling in both physiological and pathological states. In vivo sampling 
      from this site may provide important insights into which substances mediate or 
      modulate sensory transmission. To address this possibility, we have applied the 
      microdialysis technique to the guinea pig trigeminal ganglia (TG). The large size 
      and easy access of the TG in the guinea pig make it an ideal sampling site, while 
      the somatotopic organization allows for specific regions of innervation to be 
      studied at the ganglionic level. This report describes the use of microdialysis 
      probes within the TG and use recovery and analysis of substance P (SP) and 
      adenosine triphosphate (ATP) as case in points. Various physiological and 
      pharmacological manipulations can be made, for example release of peptides from 
      ganglionic neurons can be monitored in the presence or absence of inflammation in 
      the orofacial region. Microdialysis performed in the TG thus provides a valuable 
      site for recovery and measurement of a variety of extracellular substances that 
      may be integral in the processing of trigeminal sensory information.
CI  - Published by Elsevier Science B.V.
FAU - Neubert, John K
AU  - Neubert JK
AD  - Pain Neurosensory Mechanisms Branch, National Institute of Dental and 
      Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA. 
      jneubert@dir.nidcr.nih.gov
FAU - Matsuka, Yoshizo
AU  - Matsuka Y
FAU - Maidment, Nigel T
AU  - Maidment NT
FAU - Spigelman, Igor
AU  - Spigelman I
LA  - eng
GR  - DE00408/DE/NIDCR NIH HHS/United States
GR  - NS-05685/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Brain Res Protoc
JT  - Brain research. Brain research protocols
JID - 9716650
RN  - 33507-63-0 (Substance P)
RN  - 660YQ98I10 (Potassium Chloride)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/*analysis/metabolism
MH  - Animals
MH  - Facial Pain/metabolism/physiopathology
MH  - Guinea Pigs
MH  - Male
MH  - Microdialysis/instrumentation/*methods
MH  - Microelectrodes/standards
MH  - Neurons, Afferent/cytology/drug effects/*metabolism
MH  - Potassium Chloride/pharmacology
MH  - Radioimmunoassay
MH  - Substance P/*analysis/metabolism
MH  - Synaptic Transmission/physiology
MH  - Trigeminal Ganglion/cytology/drug effects/*metabolism
EDAT- 2002/11/15 04:00
MHDA- 2003/02/01 04:00
CRDT- 2002/11/15 04:00
PHST- 2002/11/15 04:00 [pubmed]
PHST- 2003/02/01 04:00 [medline]
PHST- 2002/11/15 04:00 [entrez]
AID - S1385299X02001885 [pii]
AID - 10.1016/s1385-299x(02)00188-5 [doi]
PST - ppublish
SO  - Brain Res Brain Res Protoc. 2002 Oct;10(2):102-8. doi: 
      10.1016/s1385-299x(02)00188-5.