PMID- 12435107
OWN - NLM
STAT- MEDLINE
DCOM- 20030430
LR  - 20161018
IS  - 1434-6621 (Print)
IS  - 1434-6621 (Linking)
VI  - 40
IP  - 9
DP  - 2002 Sep
TI  - The ELISPOT assay: an easily transferable method for measuring cellular responses 
      and identifying T cell epitopes.
PG  - 903-10
AB  - Characterization of human leukocyte antigen (HLA) class I restricted epitopes 
      derived from viral pathogens is imperative for formulating therapeutic 
      interventions, as well as for vaccine design and monitoring. Sensitive, easy and 
      cost-effective assays that measure the frequency of antigen-specific T 
      lymphocytes are crucial for evaluating and improving vaccines and therapies. This 
      paper reviews the ELISPOT technique that allows for quantifying HIV-specific T 
      lymphocytes at the single cell level from peripheral blood by detection of 
      antigen-induced cytokine secretion. The assay can be used successfully to 
      quantify T cell immune responses in humans infected with different pathogens and 
      to assess T cell immunogenicity of vaccines in phase I/II and III clinical 
      trials. This review focuses on the ELISPOT methodology and discusses how it can 
      be standardized and potentially used by multiple international laboratories 
      attached to clinical trial sites.
FAU - Mashishi, Tumelo
AU  - Mashishi T
AD  - AIDS Research Unit, National Institute for Communicable Diseases, Johannesburg, 
      South Africa.
FAU - Gray, Clive M
AU  - Gray CM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
PL  - Germany
TA  - Clin Chem Lab Med
JT  - Clinical chemistry and laboratory medicine
JID - 9806306
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Peptides)
RN  - 0 (Vaccines)
RN  - 0 (Viral Matrix Proteins)
SB  - IM
MH  - Enzyme-Linked Immunosorbent Assay/*methods/standards
MH  - Epitopes, T-Lymphocyte/*analysis/immunology/isolation & purification
MH  - HIV-1/genetics/immunology
MH  - Humans
MH  - Peptides/immunology
MH  - Quality Control
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Vaccines/genetics/immunology
MH  - Viral Matrix Proteins/immunology
RF  - 62
EDAT- 2002/11/19 04:00
MHDA- 2003/05/06 05:00
CRDT- 2002/11/19 04:00
PHST- 2002/11/19 04:00 [pubmed]
PHST- 2003/05/06 05:00 [medline]
PHST- 2002/11/19 04:00 [entrez]
AID - 10.1515/CCLM.2002.159 [doi]
PST - ppublish
SO  - Clin Chem Lab Med. 2002 Sep;40(9):903-10. doi: 10.1515/CCLM.2002.159.