PMID- 12437178
OWN - NLM
STAT- MEDLINE
DCOM- 20030411
LR  - 20190720
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 973
IP  - 1-2
DP  - 2002 Oct 11
TI  - Determination of poly-beta-hydroxybutyric acid in Bacillus thuringiensis by 
      capillary zone electrophoresis with indirect ultraviolet absorbance detection.
PG  - 197-202
AB  - A new capillary electrophoresis method for determining poly-beta-hydroxybutyric 
      acid (PHB) in Bacillus thuringiensis was established. Poly-beta-hydroxybutyric 
      acid in samples was hydrolyzed by sulphuric acid and neutralized by Ba(OH)2. The 
      content of produced beta-hydroxybutyrate was then determined by capillary zone 
      electrophoresis (CZE) with indirect UV detection at 254 nm. With 5 mM 
      p-hydroxybenzoate and 0.5 mM tetradecyltrimethylammonium bromide (TTAB) at pH 8.0 
      as carrier electrolyte, beta-hydroxybutyrate can be determined within 6 min. 
      Standard regression equation was made by beta-hydroxybutyrate, and the linear 
      range was 2-1000 microg/ml. The relative standard deviations (RSDs) for migration 
      time and peak area are both less than 1.0%. The detection limit for 
      beta-hydroxybutyrate was 0.2 microg/ml, which is two to three orders of magnitude 
      lower than that of the gas chromatography (GC) method. The capillary 
      electrophoresis method was successfully applied to determine 
      poly-beta-hydroxybutyric acid in fermentation broth and single colony. The added 
      standard recovery was 96%.
FAU - He, Jin
AU  - He J
AD  - College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 
      Hubei, China.
FAU - Chen, Shouwen
AU  - Chen S
FAU - Yu, Ziniu
AU  - Yu Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Hydroxybutyrates)
RN  - 0 (Polyesters)
RN  - 26063-00-3 (poly-beta-hydroxybutyrate)
SB  - IM
MH  - Bacillus thuringiensis/*chemistry
MH  - Electrophoresis, Capillary/*methods
MH  - Hydroxybutyrates/*analysis
MH  - Polyesters/*analysis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrophotometry, Ultraviolet/*methods
EDAT- 2002/11/20 04:00
MHDA- 2003/04/12 05:00
CRDT- 2002/11/20 04:00
PHST- 2002/11/20 04:00 [pubmed]
PHST- 2003/04/12 05:00 [medline]
PHST- 2002/11/20 04:00 [entrez]
AID - S0021-9673(02)01215-3 [pii]
AID - 10.1016/s0021-9673(02)01215-3 [doi]
PST - ppublish
SO  - J Chromatogr A. 2002 Oct 11;973(1-2):197-202. doi: 10.1016/s0021-9673(02)01215-3.