PMID- 12437624
OWN - NLM
STAT- MEDLINE
DCOM- 20030407
LR  - 20191106
IS  - 0902-0063 (Print)
IS  - 0902-0063 (Linking)
VI  - 16
IP  - 6
DP  - 2002 Dec
TI  - Sequential monitoring of intragraft cytokine mRNA expression in relation to 
      diastolic left ventricular wall thickness and function early after heart 
      transplantation.
PG  - 433-41
AB  - Because production of immune regulatory proteins may play a role in early graft 
      dysfunction after heart transplantation, we analyzed whether intragraft cytokine 
      messenger RNA (mRNA) expression levels are associated with diastolic left 
      ventricular function in cardiac allografts. We intensively monitored 16 cardiac 
      allograft recipients during the first 3 months after transplantation. The mRNA 
      expression levels of tumor necrosis factor (TNF-alpha), monocyte chemoattractant 
      protein-1 (MCP-1), transforming growth factor (TGF-beta), platelet derived growth 
      factor (PDGF-A), and basic fibroblast growth factor (bFGF) were measured in 
      endomyocardial biopsies (n = 123) by quantitative RT-PCR. To determine diastolic 
      allograft function, concurrent M-mode and two-dimensional Doppler echocardiograms 
      were analyzed for the following parameters: left ventricular total wall 
      thickness, maximal early and atrial mitral flow velocity, deceleration time of 
      maximal early mitral flow velocity, and isovolumetric relaxation period. During 
      the first 3 months post-transplant an overall decrease in mRNA expression levels 
      of almost all measured cytokines was observed, which paralleled an improvement in 
      diastolic left ventricular wall thickness and function. However, no 
      straightforward relationship could be found between a specific cytokine mRNA 
      expression pattern and the studied echocardiographic parameters. Our data suggest 
      that the improvement in diastolic left ventricular function is associated with a 
      general reduction of inflammation within the allograft, rather than related to a 
      specific cytokine expression pattern.
FAU - de Groot-Kruseman, Hester A
AU  - de Groot-Kruseman HA
AD  - Department of Internal Medicine, Erasmus MC, Rotterdam, The Netherlands.
FAU - Baan, Carla C
AU  - Baan CC
FAU - Hagman, Elly M
AU  - Hagman EM
FAU - Mol, Wendy M
AU  - Mol WM
FAU - Niesters, Hubert G
AU  - Niesters HG
FAU - Maat, Alex P
AU  - Maat AP
FAU - Vantrimpont, Pascal J
AU  - Vantrimpont PJ
FAU - Zondervan, Pieter E
AU  - Zondervan PE
FAU - Weimar, Willem
AU  - Weimar W
FAU - Balk, Aggie H
AU  - Balk AH
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Clin Transplant
JT  - Clinical transplantation
JID - 8710240
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Cytokines/*metabolism
MH  - Diastole/physiology
MH  - Echocardiography, Doppler
MH  - Female
MH  - Graft Rejection/*metabolism/pathology
MH  - Heart Transplantation/*physiology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Postoperative Period
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Ventricular Function, Left/*physiology
EDAT- 2002/11/20 04:00
MHDA- 2003/04/08 05:00
CRDT- 2002/11/20 04:00
PHST- 2002/11/20 04:00 [pubmed]
PHST- 2003/04/08 05:00 [medline]
PHST- 2002/11/20 04:00 [entrez]
AID - 2o068 [pii]
AID - 10.1034/j.1399-0012.2002.02068.x [doi]
PST - ppublish
SO  - Clin Transplant. 2002 Dec;16(6):433-41. doi: 10.1034/j.1399-0012.2002.02068.x.