PMID- 12438737
OWN - NLM
STAT- MEDLINE
DCOM- 20030116
LR  - 20171101
IS  - 1424-8581 (Print)
IS  - 1424-8581 (Linking)
VI  - 97
IP  - 1-2
DP  - 2002
TI  - Multicolor chromosome banding (MCB) with YAC/BAC-based probes and region-specific 
      microdissection DNA libraries.
PG  - 43-50
AB  - Multicolor chromosome banding (MCB) allows the delineation of chromosomal regions 
      with a resolution of a few megabasepairs, i.e., slightly below the size of most 
      visible chromosome bands. Based on the hybridization of overlapping 
      region-specific probe libraries, chromosomal subregions are hybridized with 
      probes that fluoresce in distinct wavelength intervals, so they can be assigned 
      predefined pseudo-colors during the digital imaging and visualization process. 
      The present study demonstrates how MCB patterns can be produced by 
      region-specific microdissection derived (mcd) libraries as well as collections of 
      yeast or bacterial artificial chromosomes (YACs and BACs, respectively). We 
      compared the efficiency of an mcd library based approach with the hybridization 
      of collections of locus-specific probes (LSP) for fluorescent banding of three 
      rather differently sized human chromosomes, i.e., chromosomes 2, 13, and 22. The 
      LSP sets were comprised of 107 probes specific for chromosome 2, 82 probes for 
      chromosome 13, and 31 probes for chromosome 22. The results demonstrated a more 
      homogeneous coverage of chromosomes and thus, more desirable banding patterns 
      using the microdissection library-based MCB. This may be related to the 
      observation that chromosomes are difficult to cover completely with YAC and/or 
      BAC clones as single-color fluorescence in situ hybridization (FISH) experiments 
      showed. Mcd libraries, on the other hand, provide high complexity probes that 
      work well as region-specific paints, but do not readily allow positioning of 
      breakpoints on genetic or physical maps as required for the positional cloning of 
      genes. Thus, combinations of mcd libraries and locus-specific large insert DNA 
      probes appear to be the most efficient tools for high-resolution cytogenetic 
      analyses.
CI  - Copyright 2002 S. Karger AG, Basel
FAU - Liehr, T
AU  - Liehr T
AD  - Institute of Human Genetics and Anthropology, University of Jena, Jena, Germany. 
      i8lith@mti-n.mti.uni-jena.de
FAU - Weise, A
AU  - Weise A
FAU - Heller, A
AU  - Heller A
FAU - Starke, H
AU  - Starke H
FAU - Mrasek, K
AU  - Mrasek K
FAU - Kuechler, A
AU  - Kuechler A
FAU - Weier, H-U G
AU  - Weier HU
FAU - Claussen, U
AU  - Claussen U
LA  - eng
GR  - 1R01 CA80792/CA/NCI NIH HHS/United States
GR  - 1R33 CA88258/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA Probes)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Chromosome Banding/*methods
MH  - Chromosome Painting
MH  - Chromosomes, Artificial, Bacterial/*genetics
MH  - Chromosomes, Artificial, Yeast/*genetics
MH  - Chromosomes, Human, Pair 13/genetics
MH  - Chromosomes, Human, Pair 2/genetics
MH  - Chromosomes, Human, Pair 22/genetics
MH  - DNA/*genetics
MH  - DNA Probes
MH  - Gene Library
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
EDAT- 2002/11/20 04:00
MHDA- 2003/01/17 04:00
CRDT- 2002/11/20 04:00
PHST- 2002/11/20 04:00 [pubmed]
PHST- 2003/01/17 04:00 [medline]
PHST- 2002/11/20 04:00 [entrez]
AID - 64043 [pii]
AID - 10.1159/000064043 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2002;97(1-2):43-50. doi: 10.1159/000064043.