PMID- 12441080
OWN - NLM
STAT- MEDLINE
DCOM- 20021219
LR  - 20190714
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 302
IP  - 2
DP  - 2002 Oct 25
TI  - Thymocyte and peripheral blood T lymphocyte subpopulation changes in piglets 
      following in utero infection with porcine reproductive and respiratory syndrome 
      virus.
PG  - 363-72
AB  - Piglets infected in utero with porcine reproductive and respiratory syndrome 
      virus (PRRSV) are born severely immunocompromised. In this article we more 
      closely examine the effects of in utero PRRSV infection on circulating and thymic 
      T cell populations. Numbers of CD4+, CD8+, and dual-positive lymphocytes were 
      quantitated in circulation and in the thymus during the 2 weeks following birth. 
      At birth we found that the number of circulating lymphocytes was suppressed by 
      60%. Lymphocyte numbers were also suppressed by 42% at day 7, but by day 14 the 
      number of lymphocytes had rebounded and was actually 47% greater than controls. 
      At birth and day 7, a drop in the number of CD4+ cells could partially explain 
      the suppression we observed, while the rebound in total lymphocyte numbers seen 
      at day 14 was due to a nearly fourfold increase in the number of circulating CD8+ 
      cells. As a result, the normal CD4+:CD8+ ratio of between 1.4 and 2.2 for 
      neonatal pigs was reduced to 0.1-0.5. The thymuses of infected piglets were found 
      to be 50% smaller than those of control pigs and were characterized by cortical 
      involution and severe cortical depletion of thymocytes. Analysis of the 
      population of thymocytes revealed that double-positive thymocytes were suppressed 
      to a greater degree than either single positive subpopulation. In addition, we 
      show that the number of thymocytes undergoing apoptosis was increased twofold in 
      piglets infected with PRRSV. Taken together, these results help explain the 
      dramatic immunosuppression observed in neonatal animals infected in utero with 
      PRRSV.
FAU - Feng, Wen-hai
AU  - Feng WH
AD  - Department of Farm Animal Health and Resource Management, College of Veterinary 
      Medicine, North Carolina State University, Raleigh, 27606, USA.
FAU - Tompkins, M B
AU  - Tompkins MB
FAU - Xu, Jin-Sheng
AU  - Xu JS
FAU - Brown, T T
AU  - Brown TT
FAU - Laster, S M
AU  - Laster SM
FAU - Zhang, He-xiao
AU  - Zhang HX
FAU - McCaw, M B
AU  - McCaw MB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Female
MH  - Infectious Disease Transmission, Vertical
MH  - Leukocytes, Mononuclear/*immunology
MH  - Lymphocyte Count
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/immunology
MH  - Pregnancy
MH  - Pregnancy, Animal
MH  - Swine
MH  - Swine Diseases/*immunology/virology
MH  - T-Lymphocyte Subsets/*immunology
MH  - Thymus Gland/cytology/*immunology
MH  - Uterus/*virology
EDAT- 2002/11/21 04:00
MHDA- 2002/12/20 04:00
CRDT- 2002/11/21 04:00
PHST- 2002/11/21 04:00 [pubmed]
PHST- 2002/12/20 04:00 [medline]
PHST- 2002/11/21 04:00 [entrez]
AID - S0042682202916508 [pii]
AID - 10.1006/viro.2002.1650 [doi]
PST - ppublish
SO  - Virology. 2002 Oct 25;302(2):363-72. doi: 10.1006/viro.2002.1650.