PMID- 12441139
OWN - NLM
STAT- MEDLINE
DCOM- 20021212
LR  - 20191210
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 281
IP  - 1
DP  - 2002 Nov 15
TI  - Nonrequirement of continuous stimulation with MCP-1 for cell migration and 
      determination of directional migration by initial stimulation with chemokine.
PG  - 157-66
AB  - Monocyte chemoattractant protein-1 (MCP-1) induces monocyte migration through 
      interaction with the MCP-1 receptor CCR2. In this report we have examined the 
      length of chemokine stimulation necessary for induction of cell migration and 
      whether continuous stimulation is required for active migration. Monocytic THP-1 
      cells prestimulated with MCP-1 for 15 to 30 min exhibited a migration response 
      after the chemokine was removed from the culture medium, indicating that a short 
      exposure to chemokine stimulation is sufficient for migration of THP-1 cells and 
      continuous stimulation is not required for active migration. A reverse gradient 
      of MCP-1 had no effect on migration after prestimulation with MCP-1. This implies 
      that cells are determined to directionally migrate by initial stimulation with 
      MCP-1. Furthermore, cell migration after prestimulation with MCP-1 was inhibited 
      by a p38 inhibitor, but not by a phosphatidylinositol 3-kinase (PI3-kinase) 
      inhibitor, indicating that p38, but not PI3-kinase, is involved in the migration 
      response after the determination of direction by initial chemokine stimulation.
FAU - Kito, Keiji
AU  - Kito K
AD  - New Product Research Laboratories II, Daiichi Pharmaceutical Company Ltd. Tokyo, 
      Japan.
FAU - Nishida, Ken ichi
AU  - Nishida Ki
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Androstadienes)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Imidazoles)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Pyridines)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - OU13V1EYWQ (SB 203580)
RN  - XVA4O219QW (Wortmannin)
SB  - IM
MH  - Androstadienes/pharmacology
MH  - Cell Communication
MH  - Cell Movement/*drug effects
MH  - Cells, Cultured/drug effects/metabolism
MH  - Chemokine CCL2/*pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - Humans
MH  - Imidazoles/pharmacology
MH  - Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism
MH  - Monocytes/*cytology
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Pyridines/pharmacology
MH  - Signal Transduction/drug effects
MH  - Wortmannin
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 2002/11/21 04:00
MHDA- 2002/12/13 04:00
CRDT- 2002/11/21 04:00
PHST- 2002/11/21 04:00 [pubmed]
PHST- 2002/12/13 04:00 [medline]
PHST- 2002/11/21 04:00 [entrez]
AID - S0014482702956636 [pii]
AID - 10.1006/excr.2002.5663 [doi]
PST - ppublish
SO  - Exp Cell Res. 2002 Nov 15;281(1):157-66. doi: 10.1006/excr.2002.5663.