PMID- 12441199
OWN - NLM
STAT- MEDLINE
DCOM- 20030922
LR  - 20190822
IS  - 0039-128X (Print)
IS  - 0039-128X (Linking)
VI  - 67
IP  - 13-14
DP  - 2002 Dec
TI  - Changes on hemostatic parameters induced by 17beta-estradiol, ethinylestradiol, 
      and the 17beta-aminoestrogen pentolame in the male Wistar rat.
PG  - 1129-35
AB  - Oral contraceptives containing estrogens increases the incidence of 
      thromboembolic events. In contrast, administration of 17beta-aminoestrogens 
      prolonged blood clotting time (BCT) in rodents. We studied the effect of 
      estradiol (E(2)), ethinylestradiol (EE) and pentolame on some screening 
      hemostatic tests. BCT was evaluated 24, 48, 72 and 96 h post-treatment. Rats 
      received subcutaneously (s.c.) for five consecutive days E(2) (0.1-1000 microg), 
      EE (1-1000 microg), pentolame (0.1-1000 microg), or vehicle (propyleneglycol 0.3 
      ml). At 48 h post-treatment E(2) (1000 microg) diminished BCT (32%, P<0.01), in 
      contrast pentolame (1000 microg) augmented BCT by 41% (P<0.01). After 72 h, E(2) 
      showed procoagulant effects with 10, 100 and 1000 microg doses (-45, -30, and 
      -21%, respectively). Significant effects on BCT of EE were observed 72 h after 
      with 1000 microg (-12%, P<0.05). Animals were treated s.c. for two consecutive 
      days with E(2) (3mg/100g), pentolame (4 mg), or vehicle (0.1 ml). BCT, bleeding 
      time (BT), platelet aggregation (PA), prothrombin time (PT), activated partial 
      thromboplastin time (APTT), thrombin time (TT) and fibrinogen concentration were 
      determined. E(2) produced a significant diminution on BCT (-20%) after 72 h 
      whereas pentolame increased BCT from 24 to 96 h (62%, maximal response at 48 h). 
      APTT and PT coagulation times of the groups treated with E(2) and pentolame were 
      lengthened (33 and 29%; 16 and 24%, respectively; P<0.05). Fibrinogen 
      concentration increased (115%, P<0.01) only in the pentolame-treated group. 
      Pentolame and E(2) produced any effects on BT and PA compared with control 
      groups, indicating that platelet function was not modified. Our results indicate 
      that E(2), EE and pentolame affects the plasmatic phase of the hemostatic 
      mechanism.
FAU - Garcia-Manzano, Aurora
AU  - Garcia-Manzano A
AD  - Departamento de Hematologia CMN, La Raza IMSS, Mexico DF, Mexico.
FAU - Gonzalez-Llaven, Jose
AU  - Gonzalez-Llaven J
FAU - Jaimez, Ruth
AU  - Jaimez R
FAU - Franco, Yanira
AU  - Franco Y
FAU - Estela Avila, Ma
AU  - Estela Avila M
FAU - Rubio-Poo, Consuelo
AU  - Rubio-Poo C
FAU - Lemini, Cristina
AU  - Lemini C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Steroids
JT  - Steroids
JID - 0404536
RN  - 0 (Amino Alcohols)
RN  - 0 (Estrenes)
RN  - 0 (Hemostatics)
RN  - 150748-24-6 (pentolame)
RN  - 423D2T571U (Ethinyl Estradiol)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Amino Alcohols/chemistry/*pharmacology
MH  - Animals
MH  - Blood Coagulation Tests
MH  - Estradiol/chemistry/*pharmacology
MH  - Estrenes/chemistry/*pharmacology
MH  - Ethinyl Estradiol/chemistry/*pharmacology
MH  - Hemostatics/*pharmacology
MH  - Male
MH  - Molecular Structure
MH  - Rats
MH  - Rats, Wistar
EDAT- 2002/11/21 04:00
MHDA- 2003/09/23 05:00
CRDT- 2002/11/21 04:00
PHST- 2002/11/21 04:00 [pubmed]
PHST- 2003/09/23 05:00 [medline]
PHST- 2002/11/21 04:00 [entrez]
AID - S0039128X02000673 [pii]
AID - 10.1016/s0039-128x(02)00067-3 [doi]
PST - ppublish
SO  - Steroids. 2002 Dec;67(13-14):1129-35. doi: 10.1016/s0039-128x(02)00067-3.