PMID- 12441677
OWN - NLM
STAT- MEDLINE
DCOM- 20030110
LR  - 20090821
IS  - 1229-845X (Print)
IS  - 1229-845X (Linking)
VI  - 3
IP  - 2
DP  - 2002 Jun
TI  - Localization of porcine reproductive and respiratory syndrome virus infection in 
      boars by in situ riboprobe hybridization.
PG  - 87-96
AB  - The capability of porcine reproductive and respiratory syndrome virus (PRRSV) to 
      be shed in semen for extended periods of time has been suggested to be a 
      principal factor for viral transmission via insemination. In attempts to gain 
      insights into the mechanism of PRRSV persistence in boars, tissue distribution 
      and sites of viral infection were investigated by in situ hybridization (ISH) 
      using digoxigenin-labeled RNA probe and the ISH results were compared with those 
      of reverse transcription-nested polymerase chain reaction (RT-nested PCR). 
      Animals were intranasally inoculated with 10(4) median tissue culture infectious 
      dose of PRRSV VR-2332 and tissues collected at different times were examined. At 
      day 7 postinfection, limited number of hybridization positive signals was 
      observed in cells within or between seminiferous tubules in the testis sections 
      while relatively abundant hybridization positive signals were observed in the 
      brain stem and tracheobronchial lymph node. At later days of infection, 
      hybridization positive signals were observed in cells within seminiferous tubules 
      with much reduced frequency. Lack of agreement with the RT-nested PCR assay 
      results in testis tissues obtained at days 14, 28, and 59 postinfection suggested 
      that PRRSV infection in the testis may be extremely restricted, and may not 
      necessarily constitute a major viral source in semen during extended periods of 
      seminal shedding.
FAU - Shin, Jin-Ho
AU  - Shin JH
AD  - Department of Clinical and Population Sciences, College of Veterinary Medicine, 
      University of Minnesota, St. Paul 55108, USA.
FAU - Molitor, Thomas W
AU  - Molitor TW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
RN  - 0 (RNA Probes)
RN  - EC 3.4.21.64 (Endopeptidase K)
SB  - IM
MH  - Animals
MH  - Brain Stem/virology
MH  - Endopeptidase K/metabolism
MH  - *In Situ Hybridization
MH  - Lymph Nodes/virology
MH  - Male
MH  - Microwaves
MH  - Porcine Reproductive and Respiratory Syndrome/transmission/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*isolation & 
      purification
MH  - RNA Probes
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Semen/virology
MH  - Seminiferous Tubules/virology
MH  - Sensitivity and Specificity
MH  - Sexually Transmitted Diseases, Viral/transmission/veterinary/virology
MH  - Swine/*virology
MH  - Testis/virology
EDAT- 2002/11/21 04:00
MHDA- 2003/01/11 04:00
CRDT- 2002/11/21 04:00
PHST- 2002/11/21 04:00 [pubmed]
PHST- 2003/01/11 04:00 [medline]
PHST- 2002/11/21 04:00 [entrez]
PST - ppublish
SO  - J Vet Sci. 2002 Jun;3(2):87-96.