PMID- 12442314
OWN - NLM
STAT- MEDLINE
DCOM- 20030207
LR  - 20091119
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 454
IP  - 3
DP  - 2002 Dec 16
TI  - Distribution of brain-derived neurotrophic factor and TrkB receptor proteins in 
      the fetal and postnatal hippocampus and cerebellum of the guinea pig.
PG  - 229-40
AB  - This study investigates the distribution of brain-derived neurotrophic factor 
      protein (BDNF) and its receptor, TrkB, during the development of hippocampus and 
      cerebellum in a long-gestation species, the guinea pig. In the granule cell 
      populations of both structures, BDNF immunoreactivity (-IR) was exclusive to 
      postmigratory, mature neurons. In dentate granule cells, TrkB-IR was coexpressed 
      with BDNF-IR, suggesting that the ligand-receptor interaction could occur by 
      means of an autocrine/paracrine mechanism. In cerebellar granule cells, TrkB-IR 
      was detected in both pre- and postmigratory cells, indicating that immature 
      neurons are also BDNF-responsive. With advancing gestational age an increase in 
      the intensity of BDNF-IR in granule cells was accompanied by concomitant 
      increases in the staining and areal growth of the associated mossy fiber layer in 
      the hippocampus, and the molecular layer in the cerebellum. The developmental 
      increase in BDNF- and TrkB-IR in the neuropil of both structures coincided with 
      periods of significant growth in all strata, indicating a role for BDNF and TrkB 
      in process outgrowth. In the hippocampus, CA2, CA3, and hilar, neurons 
      demonstrated both BDNF- and TrkB-IR during development and maturation, whereas 
      CA1 neurons showed TrkB-IR throughout this period but only transient BDNF-IR in 
      early gestation. In the fetal cerebellum, Purkinje cell bodies coexpressed 
      BDNF-IR and TrkB-IR. In the postnatal period, BDNF-IR was down-regulated but 
      TrkB-IR persisted, indicating that mature Purkinje cells might retain their 
      responsiveness to BDNF. Thus, we have demonstrated in both the hippocampus and 
      cerebellum that the spatiotemporal distribution of BDNF-IR and TrkB-IR coincides 
      with the maturation of granule cells prenatally and with significant periods of 
      neuropil growth, both prenatally and in the immediate postnatal period.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Dieni, Sandra
AU  - Dieni S
AD  - Department of Anatomy and Cell Biology, The University of Melbourne, Parkville, 
      3010 Victoria, Australia. s.dieni@anatomy.unimelb.edu.au
FAU - Rees, Sandra
AU  - Rees S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/analysis/*metabolism
MH  - Cerebellum/*chemistry/embryology/growth & development
MH  - Female
MH  - Fetus
MH  - Guinea Pigs
MH  - Hippocampus/*chemistry/embryology/growth & development
MH  - Pregnancy
MH  - Receptor, trkB/analysis/*metabolism
EDAT- 2002/11/21 04:00
MHDA- 2003/02/08 04:00
CRDT- 2002/11/21 04:00
PHST- 2002/11/21 04:00 [pubmed]
PHST- 2003/02/08 04:00 [medline]
PHST- 2002/11/21 04:00 [entrez]
AID - 10.1002/cne.10422 [doi]
PST - ppublish
SO  - J Comp Neurol. 2002 Dec 16;454(3):229-40. doi: 10.1002/cne.10422.