PMID- 12443659
OWN - NLM
STAT- MEDLINE
DCOM- 20030529
LR  - 20190728
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 21
IP  - 1-2
DP  - 2002 Nov 22
TI  - Construction and immunogenicity of recombinant Mycobacterium bovis BCG expressing 
      GP5 and M protein of porcine reproductive respiratory syndrome virus.
PG  - 21-9
AB  - Mycobacterium bovis BCG was used to express a truncated form of GP5 (lacking the 
      first 30 NH(2)-terminal residues) and M protein of porcine reproductive and 
      respiratory syndrome virus (PRRSV). The PRRSV proteins were expressed in BCG 
      under control of the mycobacterial hsp60 gene promoter either in the 
      mycobacterial cytoplasm (BCGGP5cyt and BCGMcyt) or as MT19-fusion proteins on the 
      mycobacterial surface (BCGGP5surf and BCGMsurf). Mice inoculated with BCGGP5surf 
      and BCGMsurf developed antibodies against the viral proteins at 30 days 
      post-inoculation (dpi) as detected by ELISA and Western blot. By 60 dpi, the 
      animals developed titer of neutralizing antibodies of 8. A PRRSV-specific gamma 
      interferon response was also detected in splenocytes of recombinant 
      BCG-inoculated mice at 60 and 90 dpi. These results indicate that BCG was able to 
      express antigens of PRRSV and elicit an immune response against the viral 
      proteins in mice.
FAU - Bastos, Reginaldo G
AU  - Bastos RG
AD  - Department of Veterinary and Biomedical Sciences, University of Nebraska at 
      Lincoln, Lincoln, NE 68583-0905, USA.
FAU - Dellagostin, Odir A
AU  - Dellagostin OA
FAU - Barletta, Raul G
AU  - Barletta RG
FAU - Doster, Allan R
AU  - Doster AR
FAU - Nelson, Eric
AU  - Nelson E
FAU - Osorio, Fernando A
AU  - Osorio FA
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Antibodies, Viral)
RN  - 0 (Peptides)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vaccines, DNA)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/immunology
MH  - Blotting, Western
MH  - Cloning, Molecular
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression
MH  - Genetic Vectors
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mycobacterium bovis/*genetics
MH  - Peptides/chemical synthesis/chemistry/immunology
MH  - Porcine respiratory and reproductive syndrome virus/chemistry/genetics/isolation 
      & purification
MH  - Recombinant Proteins/immunology
MH  - Swine
MH  - Vaccination
MH  - Vaccines, DNA/immunology
MH  - Viral Envelope Proteins/genetics/*immunology
MH  - Viral Matrix Proteins/genetics/*immunology
EDAT- 2002/11/22 04:00
MHDA- 2003/05/30 05:00
CRDT- 2002/11/22 04:00
PHST- 2002/11/22 04:00 [pubmed]
PHST- 2003/05/30 05:00 [medline]
PHST- 2002/11/22 04:00 [entrez]
AID - S0264410X02004437 [pii]
AID - 10.1016/s0264-410x(02)00443-7 [doi]
PST - ppublish
SO  - Vaccine. 2002 Nov 22;21(1-2):21-9. doi: 10.1016/s0264-410x(02)00443-7.