PMID- 12443832
OWN - NLM
STAT- MEDLINE
DCOM- 20030131
LR  - 20181130
IS  - 0928-8244 (Print)
IS  - 0928-8244 (Linking)
VI  - 34
IP  - 4
DP  - 2002 Dec 13
TI  - Induction of monocyte chemoattractant protein-1 by Porphyromonas gingivalis in 
      human endothelial cells.
PG  - 311-7
AB  - The association between periodontal and cardiovascular diseases could be mediated 
      by direct interaction of periodontal pathogens with cardiac tissue. In order to 
      explore this possibility, the effect of the periodontal pathogen Porphyromonas 
      gingivalis on monocyte chemoattractant protein-1 (MCP-1) production by 
      endothelial cells was investigated. When incubated with live P. gingivalis 381, 
      MCP-1 production by human umbilical vein endothelial cells (HUVEC) was potently 
      increased. Compared to the type strain 381, non-adhesive/invasive strains (W50 
      and DPG3) did not increase MCP-1 production, which was also demonstrated at the 
      mRNA level. Killed P. gingivalis 381 was much less effective than live bacteria 
      for MCP-1 induction. Treatment of HUVEC with cytochalasin D, an inhibitor of 
      endocytosis, prevented MCP-1 mRNA up-regulation by P. gingivalis 381, suggesting 
      that internalization of P. gingivalis is necessary for MCP-1 induction. In 
      conclusion, the secretion of high levels of MCP-1 resulting from interactions of 
      P. gingivalis with endothelial cells could enhance atherosclerosis progression by 
      contributing to the recruitment of monocytes.
FAU - Kang, In-Chol
AU  - Kang IC
AD  - Department of Oral Biology, State University of New York, 3435 Main Street, 
      Buffalo, NY 14214, USA.
FAU - Kuramitsu, Howard K
AU  - Kuramitsu HK
LA  - eng
GR  - DE08293/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - FEMS Immunol Med Microbiol
JT  - FEMS immunology and medical microbiology
JID - 9315554
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Arteriosclerosis/physiopathology
MH  - Bacterial Adhesion
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis
MH  - Culture Media, Conditioned
MH  - Endothelium, Vascular/cytology/metabolism/*microbiology
MH  - Humans
MH  - Porphyromonas gingivalis/*immunology/*pathogenicity
MH  - RNA, Messenger/metabolism
MH  - Up-Regulation
EDAT- 2002/11/22 04:00
MHDA- 2003/02/01 04:00
CRDT- 2002/11/22 04:00
PHST- 2002/11/22 04:00 [pubmed]
PHST- 2003/02/01 04:00 [medline]
PHST- 2002/11/22 04:00 [entrez]
AID - S0928824402004078 [pii]
AID - 10.1111/j.1574-695X.2002.tb00639.x [doi]
PST - ppublish
SO  - FEMS Immunol Med Microbiol. 2002 Dec 13;34(4):311-7. doi: 
      10.1111/j.1574-695X.2002.tb00639.x.