PMID- 12445285
OWN - NLM
STAT- MEDLINE
DCOM- 20030616
LR  - 20201222
IS  - 0105-2896 (Print)
IS  - 0105-2896 (Linking)
VI  - 188
DP  - 2002 Oct
TI  - Antitumor cytotoxic T-lymphocyte response in human lung carcinoma: identification 
      of a tumor-associated antigen.
PG  - 114-21
AB  - We have isolated several cytotoxic T lymphocyte (CTL) clones from lymphocytes 
      infiltrating a lung carcinoma of a patient with long survival. These clones 
      showed a CD3+, CD8+, CD4-, CD28- phenotype and expressed a T-cell receptor (TCR) 
      encoded either by Vbeta8-Jbeta1.5 or Vbeta22-Jbeta1.4 rearrangements. Functional 
      studies indicated that these clones mediated a high human leukocyte antigen 
      (HLA)-A2.1-restricted cytotoxic activity against the autologous tumor cell line. 
      Interestingly, TCRbeta chain gene usage indicated that CTL clones identified in 
      vitro were selectively expanded in vivo at the tumor site as compared to 
      autologous peripheral blood lymphocytes (PBL). These findings provide evidence 
      that an immune response may take place in non-small cell lung carcinoma and that 
      effector T cells may contribute to tumor regression. Further study indicated that 
      the CTL clones recognized the same decamer peptide encoded by a mutated 
      alpha-actinin-4 gene. Using tetramers of soluble HLA-A2 molecules loaded with the 
      mutated antigenic peptide, we have derived several anti-alpha-actinin-4 T-cell 
      clones from patient PBL. These CTL, recognizing a truly tumor-specific antigen, 
      may play a role in the clinical evolution of this lung cancer patient. Adoptive 
      transfer of CTL clones in a SCID/NOD mice model transplanted with autologous 
      tumor supported their antitumor effect in vivo.
FAU - Mami-Chouaib, Fathia
AU  - Mami-Chouaib F
AD  - Laboratoire Cytokines et Immunologie des tumeurs Humaines, U487 INSERM, Institut 
      Federatif de Recherche-54, Institut Gustave Roussy, Villejuif, France. 
      cfathia@igr.fr
FAU - Echchakir, Hamid
AU  - Echchakir H
FAU - Dorothee, Guillaume
AU  - Dorothee G
FAU - Vergnon, Isabelle
AU  - Vergnon I
FAU - Chouaib, Salem
AU  - Chouaib S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - England
TA  - Immunol Rev
JT  - Immunological reviews
JID - 7702118
RN  - 0 (ACTN4 protein, human)
RN  - 0 (Actn4 protein, mouse)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 11003-00-2 (Actinin)
SB  - IM
MH  - Actinin/chemistry/*immunology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigen Presentation
MH  - Antigens, Neoplasm/*immunology
MH  - Carcinoma, Non-Small-Cell Lung/*immunology/pathology
MH  - Clone Cells/immunology
MH  - Cytotoxicity, Immunologic
MH  - Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
MH  - HLA-A2 Antigen/immunology
MH  - Humans
MH  - Immunophenotyping
MH  - Immunotherapy, Adoptive
MH  - Lung Neoplasms/*immunology/pathology
MH  - Lymphocytes, Tumor-Infiltrating/*immunology
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - *Microfilament Proteins
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/chemistry/*immunology
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - T-Lymphocytes, Regulatory/immunology
MH  - Tumor Cells, Cultured/immunology
MH  - Xenograft Model Antitumor Assays
RF  - 51
EDAT- 2002/11/26 04:00
MHDA- 2003/06/17 05:00
CRDT- 2002/11/26 04:00
PHST- 2002/11/26 04:00 [pubmed]
PHST- 2003/06/17 05:00 [medline]
PHST- 2002/11/26 04:00 [entrez]
AID - imr18810 [pii]
AID - 10.1034/j.1600-065x.2002.18810.x [doi]
PST - ppublish
SO  - Immunol Rev. 2002 Oct;188:114-21. doi: 10.1034/j.1600-065x.2002.18810.x.