PMID- 12445785
OWN - NLM
STAT- MEDLINE
DCOM- 20030108
LR  - 20190710
IS  - 0022-2836 (Print)
IS  - 0022-2836 (Linking)
VI  - 324
IP  - 3
DP  - 2002 Nov 29
TI  - Asymmetric binding of histone H1 stabilizes MMTV nucleosomes and the interaction 
      of progesterone receptor with the exposed HRE.
PG  - 501-17
AB  - Packaging of mouse mammary tumor virus (MMTV) promoter sequences in nucleosomes 
      modulates access of DNA binding proteins and influences the interaction among DNA 
      bound transcription factors. Here we analyze the binding of histone H1 to MMTV 
      mononucleosomes assembled with recombinant histones and study its influence on 
      nucleosome structure and stability as well as on progesterone receptor (PR) 
      binding to the hormone responsive elements (HREs). The MMTV nucleosomes can be 
      separated into three main populations, two of which exhibited precise 
      translational positioning. Histone H1 bound preferentially to the 5' distal 
      nucleosomal DNA protecting additional 27-28 nt from digestion by micrococcal 
      nuclease. Binding of histone H1 was unaffected by prior crosslinking of protein 
      and DNA in nucleosomes with formaldehyde. Neither the translational nor the 
      rotational nucleosome positioning was altered by histone H1 binding, but the 
      nucleosomes were stabilized as judged by the kinetics of nuclease cleavage. 
      Unexpectedly, binding of recombinant PR to the exposed distal HRE-I in 
      nucleosomes was enhanced in the presence of histone H1, as demonstrated by band 
      shift and footprinting experiments. This enhanced PR affinity may contribute to 
      the reported positive effect of histone H1 on the hormonal activation of MMTV 
      reporter genes.
FAU - Vicent, Guillermo P
AU  - Vicent GP
AD  - Institut fur Molekularbiologie und Tumorforschung (IMT), Philipps-Universitat, 
      Emil-Mannkoppf-Str. 2, D-35033, Marburg, Germany.
FAU - Melia, Maria J
AU  - Melia MJ
FAU - Beato, Miguel
AU  - Beato M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Mol Biol
JT  - Journal of molecular biology
JID - 2985088R
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Histones)
RN  - 0 (Nucleosomes)
RN  - 0 (Receptors, Progesterone)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Binding Sites
MH  - Cross-Linking Reagents/chemistry
MH  - Histones/genetics/*metabolism
MH  - Humans
MH  - Mammary Tumor Virus, Mouse/genetics/*metabolism
MH  - Nucleosomes/chemistry/*metabolism
MH  - Promoter Regions, Genetic
MH  - Receptors, Progesterone/genetics/*metabolism
MH  - Recombinant Proteins/genetics/metabolism
MH  - *Response Elements
EDAT- 2002/11/26 04:00
MHDA- 2003/01/09 04:00
CRDT- 2002/11/26 04:00
PHST- 2002/11/26 04:00 [pubmed]
PHST- 2003/01/09 04:00 [medline]
PHST- 2002/11/26 04:00 [entrez]
AID - S0022283602011014 [pii]
AID - 10.1016/s0022-2836(02)01101-4 [doi]
PST - ppublish
SO  - J Mol Biol. 2002 Nov 29;324(3):501-17. doi: 10.1016/s0022-2836(02)01101-4.