PMID- 12446452
OWN - NLM
STAT- MEDLINE
DCOM- 20030718
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 101
IP  - 10
DP  - 2003 May 15
TI  - Adenosine affects expression of membrane molecules, cytokine and chemokine 
      release, and the T-cell stimulatory capacity of human dendritic cells.
PG  - 3985-90
AB  - Dendritic cells (DCs) express functional purinergic type 1 receptors, but the 
      effects of adenosine in these antigen-presenting cells have been only marginally 
      investigated. Here, we further characterized the biologic activity of adenosine 
      in immature DCs (iDCs) and lipopolysaccharide (LPS)-matured DCs (mDCs). Chronic 
      stimulation with adenosine enhanced the macropinocytotic activity and the 
      membrane expression of CD80, CD86, major histocompatibility complex (MHC) class 
      I, and HLA-DR molecules on iDCs. Adenosine also increased LPS-induced CD54, CD80, 
      MHC class I, and HLA-DR molecule expression in mDCs. In addition, adenosine 
      dose-dependently inhibited tumor necrosis factor alpha and interleukin-12 (IL-12) 
      release, whereas it enhanced the secretion of IL-10 from mDCs. The use of 
      selective receptor agonists revealed that the modulation of the cytokine and 
      cell-surface marker profile was due to activation of A(2) adenosine receptor. 
      Functionally, adenosine reduced the allostimulatory capacity of iDCs, but not of 
      mDCs. More important, DCs matured in the presence of adenosine had a reduced 
      capacity to induce T helper 1 (Th1) polarization of naive CD4(+) T lymphocytes. 
      Finally, adenosine augmented the release of the chemokine CCL17 and inhibited 
      CXCL10 production by mDCs. In aggregate, the results provide initial evidence 
      that adenosine diminishes the capacity of DCs to initiate and amplify Th1 immune 
      responses.
FAU - Panther, Elisabeth
AU  - Panther E
AD  - Department of Experimental Dermatology and Pneumology, University of Freiburg, 
      Germany.
FAU - Corinti, Silvia
AU  - Corinti S
FAU - Idzko, Marco
AU  - Idzko M
FAU - Herouy, Yared
AU  - Herouy Y
FAU - Napp, Matthias
AU  - Napp M
FAU - la Sala, Andrea
AU  - la Sala A
FAU - Girolomoni, Giampiero
AU  - Girolomoni G
FAU - Norgauer, Johannes
AU  - Norgauer J
LA  - eng
PT  - Journal Article
DEP - 20021121
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 120442-40-2 (CGS 24012)
RN  - 152918-18-8 (N(6)-(3-iodobenzyl)-5'-N-methylcarboxamidoadenosine)
RN  - 187348-17-0 (Interleukin-12)
RN  - K72T3FS567 (Adenosine)
SB  - IM
MH  - Adenosine/*analogs & derivatives/*pharmacology
MH  - Antigens, CD/*blood
MH  - Cells, Cultured
MH  - Chemokines/*blood
MH  - Cytokines/*blood
MH  - Dendritic Cells/drug effects/*immunology
MH  - Endocytosis/drug effects
MH  - Flow Cytometry
MH  - Histocompatibility Antigens Class I/blood
MH  - Humans
MH  - Interleukin-12/blood
MH  - Kinetics
MH  - Lipopolysaccharides/pharmacology
MH  - Lymphocyte Activation
MH  - T-Lymphocytes/drug effects/*immunology
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2002/11/26 04:00
MHDA- 2003/07/19 05:00
CRDT- 2002/11/26 04:00
PHST- 2002/11/26 04:00 [pubmed]
PHST- 2003/07/19 05:00 [medline]
PHST- 2002/11/26 04:00 [entrez]
AID - S0006-4971(20)44448-9 [pii]
AID - 10.1182/blood-2002-07-2113 [doi]
PST - ppublish
SO  - Blood. 2003 May 15;101(10):3985-90. doi: 10.1182/blood-2002-07-2113. Epub 2002 
      Nov 21.