PMID- 12447651
OWN - NLM
STAT- MEDLINE
DCOM- 20030428
LR  - 20151119
IS  - 1432-9417 (Print)
IS  - 1432-9417 (Linking)
VI  - 6
IP  - 6
DP  - 2002 Nov
TI  - [Opportunities and chances for tissue chip microarrays in head and neck surgery. 
      A novel technique for the rapid evaluation of potentially novel biomarkers].
PG  - 394-401
AB  - Oral squamous cell carcinomas (OSCC) are malignant tumors with a poor prognosis 
      and low long-term survival rates, even when using modern adjuvant and neoadjuvant 
      therapy forms in addition to surgery. For the clinical estimation of each tumor, 
      it is necessary to define stage-dependent molecular and/or cellular parameters as 
      it is known that OSCC develop along a multistep pathway including the loss of 
      tumor suppressor genes and the amplification of oncogenes which result in changes 
      in protein expression. In order to establish a reliable pattern of molecular and 
      cellular biomarkers, a large number of tumor specimens from different stages of 
      the disease need to be analysed. In this study, biopsies of a collective of 293 
      OSCC in different stages were screened with the novel technique of tissue chip 
      microarrays by fluorescence in situ hybridization (FISH) and immunohistochemistry 
      (IHC). FISH-analysis was performed on the oncogene cyclin D1 and IHC-analysis on 
      the proteins cyclin D1, p53, p16, cdk4, bcl2, mdm2 and rb. Tissue chip technology 
      was shown to facilitate rapid screening for molecular and cellular alterations in 
      different stages of OSCC and revealed reliable and reproducible results that may 
      allow the definition of a multistep pathway model for tumor progression in OSCC.
FAU - Hofele, C
AU  - Hofele C
AD  - Klinik und Poliklinik fur Mund-, Kiefer- und Gesichtschirurgie, Universitat 
      Heidelberg, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany. 
      chofele@med.uni-heidelberg.de
FAU - Joos, S
AU  - Joos S
FAU - Flechtenmacher, C
AU  - Flechtenmacher C
FAU - Bosch, F X
AU  - Bosch FX
FAU - Lichter, P
AU  - Lichter P
FAU - Muhling, J
AU  - Muhling J
FAU - Freier, K
AU  - Freier K
LA  - ger
PT  - English Abstract
PT  - Journal Article
TT  - Moglichkeiten und chancen der Gewebechiptechnologie bei Kopf-Hals-Tumoren. Eine 
      neue Technik zur schnellen Analyse von potenziellen Tumormarkern.
DEP - 20020702
PL  - Germany
TA  - Mund Kiefer Gesichtschir
JT  - Mund-, Kiefer- und Gesichtschirurgie : MKG
JID - 9716576
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Aged
MH  - Biomarkers, Tumor/analysis/*genetics
MH  - Biopsy/instrumentation
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Transformation, Neoplastic/genetics/pathology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Mouth Mucosa/pathology
MH  - Mouth Neoplasms/*genetics/pathology
MH  - Neoplasm Staging
MH  - Oligonucleotide Array Sequence Analysis/*instrumentation
MH  - Prognosis
EDAT- 2002/11/26 04:00
MHDA- 2003/04/29 05:00
CRDT- 2002/11/26 04:00
PHST- 2002/11/26 04:00 [pubmed]
PHST- 2003/04/29 05:00 [medline]
PHST- 2002/11/26 04:00 [entrez]
AID - 10.1007/s10006-002-0412-5 [doi]
PST - ppublish
SO  - Mund Kiefer Gesichtschir. 2002 Nov;6(6):394-401. doi: 10.1007/s10006-002-0412-5. 
      Epub 2002 Jul 2.