PMID- 12450852
OWN - NLM
STAT- MEDLINE
DCOM- 20030213
LR  - 20240322
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 68
IP  - 12
DP  - 2002 Dec
TI  - Direct identification in food samples of Listeria spp. and Listeria monocytogenes 
      by molecular methods.
PG  - 6273-82
AB  - A new molecular approach for the detection and identification of Listeria spp. 
      and Listeria monocytogenes in food is presented here. The method is based on the 
      PCR amplification of a fragment of the iap gene from the five species belonging 
      to the genus and on the analysis of the PCR products obtained by denaturing 
      gradient gel electrophoresis (DGGE). The protocol was first optimized by using 
      strains from international collections. Based on the differences present in the 
      sequences amplified, it was possible to obtain species-specific DGGE migration 
      that allowed fast and easy identification of L. monocytogenes, L. innocua, L. 
      welshimeri, L. seeligeri, and L. ivanovii. Moreover, for L. monocytogenes 
      serotypes, partial differentiation was possible. The optimized protocol was used 
      for identification of Listeria strains traditionally isolated from food and for 
      direct detection and identification of Listeria members in food after an 
      overnight enrichment. Identification of 48 food isolates and direct detection of 
      Listeria spp. in 73 food samples show the potential of the method that can be 
      used as a fast screening test to investigate the presence of Listeria spp. and L. 
      monocytogenes in food.
FAU - Cocolin, Luca
AU  - Cocolin L
AD  - Dipartimento Scienze degli Alimenti, Universita degli studi di Udine, Italy. 
      luca.cocolin@dsa.uniud.it
FAU - Rantsiou, Kalliopi
AU  - Rantsiou K
FAU - Iacumin, Lucilla
AU  - Iacumin L
FAU - Cantoni, Carlo
AU  - Cantoni C
FAU - Comi, Giuseppe
AU  - Comi G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (60 kDa protein, Listeria monocytogenes)
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Bacterial Proteins/*genetics
MH  - DNA, Bacterial/analysis
MH  - *Food Microbiology
MH  - Listeria/*isolation & purification
MH  - Listeria monocytogenes/*isolation & purification
MH  - Polymerase Chain Reaction
PMC - PMC134388
EDAT- 2002/11/27 04:00
MHDA- 2003/02/14 04:00
PMCR- 2002/12/01
CRDT- 2002/11/27 04:00
PHST- 2002/11/27 04:00 [pubmed]
PHST- 2003/02/14 04:00 [medline]
PHST- 2002/11/27 04:00 [entrez]
PHST- 2002/12/01 00:00 [pmc-release]
AID - 0551 [pii]
AID - 10.1128/AEM.68.12.6273-6282.2002 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2002 Dec;68(12):6273-82. doi: 
      10.1128/AEM.68.12.6273-6282.2002.