PMID- 12451139
OWN - NLM
STAT- MEDLINE
DCOM- 20021223
LR  - 20200225
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 22
IP  - 23
DP  - 2002 Dec 1
TI  - Cellular mechanisms regulating activity-dependent release of native brain-derived 
      neurotrophic factor from hippocampal neurons.
PG  - 10399-407
AB  - Brain-derived neurotrophic factor (BDNF) plays a critical role in 
      activity-dependent modifications of neuronal connectivity and synaptic strength, 
      including establishment of hippocampal long-term potentiation (LTP). To shed 
      light on mechanisms underlying BDNF-dependent synaptic plasticity, the present 
      study was undertaken to characterize release of native BDNF from newborn rat 
      hippocampal neurons in response to physiologically relevant patterns of 
      electrical field stimulation in culture, including tonic stimulation at 5 Hz, 
      bursting stimulation at 25 and 100 Hz, and theta-burst stimulation (TBS). Release 
      was measured using the ELISA in situ technique, developed in our laboratory to 
      quantify secretion of native BDNF without the need to first overexpress the 
      protein to nonphysiological levels. Each stimulation protocol resulted in a 
      significant increase in BDNF release that was tetrodotoxin sensitive and occurred 
      in the absence of glutamate receptor activation. However, 100 Hz tetanus and TBS, 
      stimulus patterns that are most effective in inducing hippocampal LTP, were 
      significantly more effective in releasing native BDNF than lower-frequency 
      stimulation. For all stimulation protocols tested, removal of extracellular 
      calcium, or blockade of N-type calcium channels, prevented BDNF release. 
      Similarly, depletion of intracellular calcium stores with thapsigargin and 
      treatment with dantrolene, an inhibitor of calcium release from 
      caffeine-ryanodine-sensitive stores, markedly inhibited activity-dependent BDNF 
      release. Our results indicate that BDNF release can encode temporal features of 
      hippocampal neuronal activity. The dual requirement for calcium influx through 
      N-type calcium channels and calcium mobilization from intracellular stores 
      strongly implicates a role for calcium-induced calcium release in 
      activity-dependent BDNF secretion.
FAU - Balkowiec, Agnieszka
AU  - Balkowiec A
AD  - Department of Neurosciences, Case Western Reserve University School of Medicine, 
      Cleveland, Ohio 44106, USA.
FAU - Katz, David M
AU  - Katz DM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Calcium Channels, N-Type)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Sodium Channels)
RN  - 15662-33-6 (Ryanodine)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - 660YQ98I10 (Potassium Chloride)
RN  - F64QU97QCR (Dantrolene)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Action Potentials/physiology
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/metabolism
MH  - Calcium/metabolism
MH  - Calcium Channel Blockers/pharmacology
MH  - Calcium Channels, N-Type/drug effects
MH  - Cells, Cultured
MH  - Dantrolene/pharmacology
MH  - Electric Stimulation/methods
MH  - Enzyme Inhibitors/pharmacology
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Hippocampus/cytology/drug effects/*metabolism
MH  - Long-Term Potentiation/physiology
MH  - Neuronal Plasticity/drug effects/physiology
MH  - Neurons/cytology/drug effects/*metabolism
MH  - Potassium Chloride/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Ryanodine/pharmacology
MH  - Sodium Channels/metabolism
MH  - Synaptic Transmission/drug effects/physiology
MH  - Tetrodotoxin/pharmacology
MH  - Time Factors
PMC - PMC6758764
EDAT- 2002/11/27 04:00
MHDA- 2002/12/27 04:00
PMCR- 2003/06/01
CRDT- 2002/11/27 04:00
PHST- 2002/11/27 04:00 [pubmed]
PHST- 2002/12/27 04:00 [medline]
PHST- 2002/11/27 04:00 [entrez]
PHST- 2003/06/01 00:00 [pmc-release]
AID - 22/23/10399 [pii]
AID - 7072 [pii]
AID - 10.1523/JNEUROSCI.22-23-10399.2002 [doi]
PST - ppublish
SO  - J Neurosci. 2002 Dec 1;22(23):10399-407. doi: 10.1523/JNEUROSCI.22-23-10399.2002.