PMID- 12452867
OWN - NLM
STAT- MEDLINE
DCOM- 20030213
LR  - 20221109
IS  - 0007-0963 (Print)
IS  - 0007-0963 (Linking)
VI  - 147
IP  - 6
DP  - 2002 Dec
TI  - Dendritic cells and apoptosis in mycosis fungoides.
PG  - 1171-9
AB  - BACKGROUND: The existence of an effective antitumour immune response in mycosis 
      fungoides (MF) has been shown by the isolation of tumour-specific T-cell clones 
      from such patients. Dendritic cells (DCs) are considered crucial for the 
      induction of immunity, including resistance to tumours. Apoptotic tumour cells 
      are a major source for tumour antigens processed and presented by DCs via 
      cross-presentation. The production of interleukin (IL)-10 by MF tumour cells is 
      acknowledged and may block DCs maturation leading to tumour tolerance. 
      OBJECTIVES: Cross-presentation of apoptotic tumour cells by DCs will induce 
      immunity if the DCs mature, but tolerance if maturation does not occur. We now 
      further characterize the DCs in skin infiltrates of patch/plaque-stage MF (PS) 
      and tumour-stage MF (TS) in situ. Secondly, we demonstrate apoptosis in MF 
      infiltrates in situ and analyse the association of apoptotic cells to immature 
      DCs, mature DCs and IL-10-positive cells. METHODS: Immunohistochemical staining 
      (single, double, triple) employing novel markers specific for immature and mature 
      DCs, IL-10 and a terminal deoxynucleotidyl transferase-mediated deoxyuridine 
      triphosphate nick end labelling (TUNEL) test were done on representative skin 
      biopsies from PS and TS. RESULTS: In PS, the immature DCs are mostly lag/langerin 
      + Langerhans cells (LCs). In the epidermis of PS, LCs predominate over fully 
      mature DCs (non-LC type, CD83+, DC-lamp+). In the dermis of PS and TS, equal 
      numbers of mature and immature (CD1a+, CD1c+) DCs are densely interspersed 
      between the lymphocytic infiltrate. In TS, immature DCs mostly lack lag or 
      langerin expression. Immature DCs with incorporated apoptotic cells were found 
      rarely in PS but increasingly in TS. By triple staining in situ we could now show 
      that strongly IL-10+ cells frequently surround immature DCs, some of them with 
      incorporated apoptotic cells. CONCLUSIONS; DCs in MF perform a dual role, namely 
      induction and maintenance of antitumour immunity, or, under less favourable 
      circumstances such as production of IL-10 downregulation of antitumour immunity. 
      The latter condition was mainly seen in TS, possibly explaining disease 
      progression. Further in vitro studies are now required illuminating the role of 
      DCs for the antitumour immune response in MF.
FAU - Luftl, M
AU  - Luftl M
AD  - Department of Dermatology, University Hospital of Erlangen, Hartmannstr. 14, 
      D-91052 Erlangen, Germany. matthias.lueftl@derma.imed.uni-erlangen.de
FAU - Feng, A
AU  - Feng A
FAU - Licha, E
AU  - Licha E
FAU - Schuler, G
AU  - Schuler G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Dermatol
JT  - The British journal of dermatology
JID - 0004041
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Apoptosis/*immunology
MH  - Cell Differentiation
MH  - Dendritic Cells/*pathology
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Interleukin-10/analysis
MH  - Langerhans Cells/pathology
MH  - Mycosis Fungoides/*immunology/pathology
MH  - Skin Neoplasms/*immunology/pathology
EDAT- 2002/11/28 04:00
MHDA- 2003/02/14 04:00
CRDT- 2002/11/28 04:00
PHST- 2002/11/28 04:00 [pubmed]
PHST- 2003/02/14 04:00 [medline]
PHST- 2002/11/28 04:00 [entrez]
AID - 4994 [pii]
AID - 10.1046/j.1365-2133.2002.04994.x [doi]
PST - ppublish
SO  - Br J Dermatol. 2002 Dec;147(6):1171-9. doi: 10.1046/j.1365-2133.2002.04994.x.