PMID- 12452949
OWN - NLM
STAT- MEDLINE
DCOM- 20030505
LR  - 20190916
IS  - 1364-5072 (Print)
IS  - 1364-5072 (Linking)
VI  - 93
IP  - 6
DP  - 2002
TI  - Sterilization of plastic containers using electron beam irradiation directed 
      through the opening.
PG  - 937-43
AB  - AIMS: Studies were performed to demonstrate the efficacy of a novel electron beam 
      irradiation system for the on-line sterilization of polymeric containers using 
      energetic electrons directed through the container opening. METHODS AND RESULTS: 
      The distribution of dosage delivered during conveyance beneath the electron beam 
      treatment system was determined for two sizes (8 or 16 ounces) of high-density 
      polyethylene (HDPE) blow-moulded bottles. The biological effects of treatment 
      were then determined using Bacillus pumilus ATCC 27142 spores inoculated as 10 
      microl droplets dried (i) onto the surface of flat coupons of the high-density 
      polyethylene bottle material, and (ii) onto the region of lowest delivered dose 
      (the bottom side wall) within each bottle. The inactivation obtained was 
      determined by examining the level of survival after swab recovery of the 
      inoculated spores, with reference to the level recovered from untreated control 
      samples. CONCLUSIONS: The inactivation of B. pumilus spores during treatment was 
      reproducible and proportional to the applied dose. In each instance, the 
      logarithm of the surviving fraction of spores was well fitted by linear 
      regression models and in good general agreement with values reported for 
      inactivation of the same organism using gamma irradiation. SIGNIFICANCE AND 
      IMPACT OF THE STUDY: The results of this work demonstrate the potential of a new 
      physical method capable of high throughput for in-line sterilization of polymeric 
      containers. The ability of the process to eliminate the washing step involved 
      with traditional chemical sterilants, such as hydrogen peroxide, greatly reduces 
      the size and environmental impact of these systems.
FAU - Cleghorn, D A
AU  - Cleghorn DA
AD  - Electron Processing Systems, Inc, North Billerica, MA 01862, USA.
FAU - Dunn, J
AU  - Dunn J
FAU - Nablo, S V
AU  - Nablo SV
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Plastics)
SB  - IM
MH  - Bacillus/radiation effects
MH  - *Electrons
MH  - Food Microbiology
MH  - *Food Packaging
MH  - *Plastics
MH  - Spores, Bacterial
MH  - Sterilization/*methods
EDAT- 2002/11/28 04:00
MHDA- 2003/05/06 05:00
CRDT- 2002/11/28 04:00
PHST- 2002/11/28 04:00 [pubmed]
PHST- 2003/05/06 05:00 [medline]
PHST- 2002/11/28 04:00 [entrez]
AID - 1777 [pii]
AID - 10.1046/j.1365-2672.2002.01777.x [doi]
PST - ppublish
SO  - J Appl Microbiol. 2002;93(6):937-43. doi: 10.1046/j.1365-2672.2002.01777.x.