PMID- 12453056
OWN - NLM
STAT- MEDLINE
DCOM- 20030305
LR  - 20190815
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 16
IP  - 10
DP  - 2002 Nov
TI  - Effects of dark rearing on phosphorylation of neurotrophin Trk receptors.
PG  - 1925-30
AB  - Total lack of visual experience (dark rearing, DR) is known to affect development 
      of mammalian visual cortex (VC) and to prolong the critical period of visual 
      cortical plasticity. Neurotrophins (NTs) have been proposed to play a relevant 
      role in activity dependent processes important for the final shaping of cortical 
      visual connections. Neurotrophin supply or antagonism of endogenous NT action 
      profoundly affect visual cortical development and plasticity; in particular, 
      exogenous supply of NTs counteracts DR effects on VC development. However, the 
      effects of DR on NT expression are still debated and mounting evidence reports a 
      mismatch between BDNF mRNA and protein expression in DR animals. To gain insight 
      into the effects of DR on expression of nerve growth factor (NGF) and the 
      functional state of NT signalling pathways, we assessed the phosphorylation state 
      of Trk receptors in light-reared animals (LR), in dark-reared animals (DR), in DR 
      animals briefly exposed to light and in DR animals with exogenous supply of NTs 
      [NGF, brain-derived neurotrophic factor (BDNF) and NT-4] in the VC. We report 
      that DR increases the expression of NGF but reduces the phosphorylation of TrkA 
      and TrkB receptors with respect to LR; normal phosphorylation is rapidly rescued 
      by a brief exposure to light. Exogenous supply of NGF, BDNF or NT4 in DR animals 
      also rescues the phosphorylation of their receptors.
FAU - Viegi, Alessandro
AU  - Viegi A
AD  - Scuola Normale Superiore, p.zza Dei Cavalieri, 7, 56126, Pisa, Italy. 
      viegi@in.pi.cnr.it
FAU - Cotrufo, Tiziana
AU  - Cotrufo T
FAU - Berardi, Nicoletta
AU  - Berardi N
FAU - Mascia, Laura
AU  - Mascia L
FAU - Maffei, Lamberto
AU  - Maffei L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptor, trkA)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Darkness/*adverse effects
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunoblotting
MH  - Nerve Growth Factor/*metabolism/pharmacology
MH  - Nerve Growth Factors/pharmacology
MH  - Phosphorylation/drug effects
MH  - Photic Stimulation
MH  - Precipitin Tests
MH  - Rats
MH  - Rats, Long-Evans
MH  - Receptor, trkA/drug effects/*metabolism
MH  - Receptor, trkB/drug effects/*metabolism
MH  - *Sensory Deprivation
MH  - Signal Transduction/drug effects/physiology
MH  - Up-Regulation/drug effects
MH  - Visual Cortex/drug effects/*metabolism
EDAT- 2002/11/28 04:00
MHDA- 2003/03/06 04:00
CRDT- 2002/11/28 04:00
PHST- 2002/11/28 04:00 [pubmed]
PHST- 2003/03/06 04:00 [medline]
PHST- 2002/11/28 04:00 [entrez]
AID - 2270 [pii]
AID - 10.1046/j.1460-9568.2002.02270.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2002 Nov;16(10):1925-30. doi: 10.1046/j.1460-9568.2002.02270.x.