PMID- 12453825
OWN - NLM
STAT- MEDLINE
DCOM- 20030321
LR  - 20171116
IS  - 0193-1849 (Print)
IS  - 0193-1849 (Linking)
VI  - 284
IP  - 3
DP  - 2003 Mar
TI  - Influence of growth hormone on bone marrow adipogenesis in hypophysectomized 
      rats.
PG  - E566-73
AB  - The hypophysectomized rat has been used as a model to study the effects of growth 
      hormone deficiency on bone. Here, we have investigated the influence of growth 
      hormone administration to hypophysectomized rats (HX) for 6 wk on accumulation of 
      triglycerides in bone marrow and on the differentiation of primary marrow stromal 
      cells into adipocytes under in vitro conditions. We found that hypophysectomy 
      significantly increased triglyceride concentration in bone marrow, which was 
      attenuated by growth hormone administration. Primary bone marrow stromal cells 
      derived from HX rats also had more adipocytes at confluence compared with growth 
      hormone-treated hypophysectomized (GH) rats. When stimulated with 
      3-isobutyl-1-methylxanthine plus dexamethasone (IBMX-Dex), preadipocyte colony 
      counts increased more significantly in GH rats. Markers of adipocyte 
      differentiation were higher in HX than in control or GH rats at confluence. 
      However, after stimulation with IBMX-Dex, increased expression of markers was 
      seen in GH compared with HX rats. In conclusion, growth hormone administration to 
      hypophysectomized rats attenuated triglyceride accumulation in bone marrow and 
      inhibited the differentiation of stromal cells into adipocytes in vitro.
FAU - Appiagyei-Dankah, Yaw
AU  - Appiagyei-Dankah Y
AD  - Division of Pediatric Endocrinology, Winthrop-University Hospital, Mineola, NY 
      11501, USA.
FAU - Tapiador, Carmen D
AU  - Tapiador CD
FAU - Evans, Jodi F
AU  - Evans JF
FAU - Castro-Magana, Mariano
AU  - Castro-Magana M
FAU - Aloia, John F
AU  - Aloia JF
FAU - Yeh, James K
AU  - Yeh JK
LA  - eng
PT  - Journal Article
DEP - 20021126
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (Biomarkers)
RN  - 0 (Triglycerides)
RN  - 9002-72-6 (Growth Hormone)
SB  - IM
MH  - Adipocytes/*cytology/physiology
MH  - Animals
MH  - Biomarkers
MH  - Bone Marrow Cells/*cytology/*metabolism
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Cellular Senescence/physiology
MH  - Growth Hormone/*pharmacology
MH  - *Hypophysectomy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stem Cells/cytology
MH  - Stromal Cells/cytology
MH  - Triglycerides/*metabolism
EDAT- 2002/11/28 04:00
MHDA- 2003/03/22 04:00
CRDT- 2002/11/28 04:00
PHST- 2002/11/28 04:00 [pubmed]
PHST- 2003/03/22 04:00 [medline]
PHST- 2002/11/28 04:00 [entrez]
AID - 00213.2002 [pii]
AID - 10.1152/ajpendo.00213.2002 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2003 Mar;284(3):E566-73. doi: 
      10.1152/ajpendo.00213.2002. Epub 2002 Nov 26.