PMID- 12454045
OWN - NLM
STAT- MEDLINE
DCOM- 20021212
LR  - 20061115
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 43
IP  - 12
DP  - 2002 Dec
TI  - Effects of muller glia on cell survival and neuritogenesis in adult porcine 
      retina in vitro.
PG  - 3735-43
AB  - PURPOSE: To examine the effects of glia-derived and brain-derived neurotrophic 
      factors on survival and morphology of cultured retinal ganglion cells (RGCs) from 
      adult porcine retina. METHODS: Adult porcine retinas were dissociated and 
      cultured in different conditions: (1) on laminin- and poly-D-lysine-coated 
      coverslips in chemically defined medium (CDM); (2) on laminin- and 
      poly-D-lysine-coated coverslips in CDM supplemented with brain-derived 
      neurotrophic factor (BDNF); (3) in confluent monolayer cultures of retinal Muller 
      glia (RMG) in CDM; (4) in 1-day cultures of RMG in CDM; (5) in fixed RMG cultures 
      in CDM; and (6) in laminin-poly-D-lysine substrate in conditioned medium obtained 
      from RMG. RGCs were classified on the basis of the size, number of neurites, and 
      length of the neurites, and the survival of the RGCs was assayed after each 
      treatment. RESULTS: Confluent RMG substrates and RMG-conditioned medium 
      significantly increased the survival of cultured porcine RGCs. Moreover, these 
      two conditions increased the size of the RGCs and enhanced growth and elongation 
      of the neurite. Addition of BDNF to the culture medium or use of 1-day cultured 
      RMG as a substrate did not modify survival but increased the size, neurite 
      number, and neurite length in the RCGs. CONCLUSIONS: These findings demonstrate 
      that factor(s) secreted by RMG exert beneficial effects on survival of adult RGCs 
      and neurite regeneration in vitro and may constitute effective agent(s) for 
      neuroprotection of RGC.
FAU - Garcia, Monica
AU  - Garcia M
AD  - Department of Cellular Biology, Faculty of Medicine, University of the Basque 
      Country, Vizcaya, Spain.
FAU - Forster, Valerie
AU  - Forster V
FAU - Hicks, David
AU  - Hicks D
FAU - Vecino, Elena
AU  - Vecino E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Size
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Fluorescent Antibody Technique, Indirect
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Nerve Growth Factors/*pharmacology
MH  - Neurites/physiology
MH  - Neuroglia/*physiology
MH  - Retinal Ganglion Cells/*cytology/drug effects
MH  - Swine
EDAT- 2002/11/28 04:00
MHDA- 2002/12/13 04:00
CRDT- 2002/11/28 04:00
PHST- 2002/11/28 04:00 [pubmed]
PHST- 2002/12/13 04:00 [medline]
PHST- 2002/11/28 04:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2002 Dec;43(12):3735-43.