PMID- 12454046
OWN - NLM
STAT- MEDLINE
DCOM- 20021212
LR  - 20081121
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 43
IP  - 12
DP  - 2002 Dec
TI  - Protective effect against ischemia and light damage of iris pigment epithelial 
      cells transfected with the BDNF gene.
PG  - 3744-53
AB  - PURPOSE: Brain-derived neurotrophic factor (BDNF) has been reported to protect 
      retinal neurons against ischemia and light-induced damage. In the current study, 
      the BDNF gene was transfected into iris pigment epithelial (IPE) cells of 
      Long-Evans rats, and the neuroprotective ability of the IPE cells transfected 
      with the BDNF gene against N-methyl-D-aspartate (NMDA)-induced neuroretinal cell 
      death and against phototoxic damage was examined. METHODS: The level of BDNF mRNA 
      and protein expressed in the transfected cells was determined by reverse 
      transcription-polymerase chain reaction (RT-PCR) and by sandwich enzyme-linked 
      immunosorbent assay (ELISA). The neuroprotective effects were determined by 
      culturing BDNF gene-transfected IPE cells or nontransfected cells with 
      neuroretinal cells in the presence of NMDA. The neuroprotective activity was also 
      evaluated for the damage induced by constant exposure to light on the 
      photoreceptors by transplanting BDNF gene-transfected IPE cells into the 
      subretinal region of the superior half of the eye. RESULTS: BDNF gene-transfected 
      IPE cells expressed higher levels of BDNF mRNA and protein than did 
      nontransfected IPE cells. A significant increase in the protection against NMDA 
      was observed in the neuroretinal cells cultured with BDNF-transfected IPE cells 
      than in those cultured with nontransfected IPE cells (P = 0.0029) or with 
      nontreated cells (P = 0.0010). The effect was partially attenuated by the 
      addition of anti-BDNF antibody. Significant photoreceptor cell protection against 
      injury from constant light was also observed by the subretinal transplantation of 
      BDNF-transfected IPE cells when compared with those receiving transplants of 
      nontransfected cells or vehicle injection. CONCLUSIONS: BDNF-transfected IPE 
      cells demonstrated a neuronal rescue effect in both in vitro and in vivo 
      experiments. IPE cells may be a potential source for autologous cell 
      transplantation for some retinal diseases, and the transfection of the genes of 
      neurotrophic factors into the transplanted cell may be a useful tool for 
      delivering these factors to the retina.
FAU - Kano, Tetsuya
AU  - Kano T
AD  - Department of Ophthalmology, Tohoku University, School of Medicine, Miyagi, 
      Japan. t-kano@oph.med.tohoku.ac.jp
FAU - Abe, Toshiaki
AU  - Abe T
FAU - Tomita, Hiroshi
AU  - Tomita H
FAU - Sakata, Tsuneaki
AU  - Sakata T
FAU - Ishiguro, Sei-ichi
AU  - Ishiguro S
FAU - Tamai, Makoto
AU  - Tamai M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 6384-92-5 (N-Methylaspartate)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Cell Survival
MH  - Coculture Techniques
MH  - Cytoprotection
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunoenzyme Techniques
MH  - Iris/cytology/*metabolism
MH  - Ischemia/metabolism/pathology/*prevention & control
MH  - Light
MH  - Male
MH  - N-Methylaspartate/toxicity
MH  - Photoreceptor Cells, Vertebrate/drug effects/*radiation effects
MH  - Pigment Epithelium of Eye/cytology/*physiology
MH  - RNA, Messenger/metabolism
MH  - Radiation Injuries, Experimental/metabolism/pathology/*prevention & control
MH  - Rats
MH  - Rats, Long-Evans
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
MH  - Retinal Degeneration/metabolism/pathology/*prevention & control
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Transfection
EDAT- 2002/11/28 04:00
MHDA- 2002/12/13 04:00
CRDT- 2002/11/28 04:00
PHST- 2002/11/28 04:00 [pubmed]
PHST- 2002/12/13 04:00 [medline]
PHST- 2002/11/28 04:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2002 Dec;43(12):3744-53.