PMID- 12454272
OWN - NLM
STAT- MEDLINE
DCOM- 20030604
LR  - 20210222
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 43
IP  - 12
DP  - 2002 Dec
TI  - Increased lipolysis in transgenic animals overexpressing the epithelial fatty 
      acid binding protein in adipose cells.
PG  - 2105-11
AB  - Fatty acid binding proteins (FABPs) are low-molecular-mass, soluble, 
      intracellular lipid carriers. Previous studies on adipocytes from adipocyte fatty 
      acid binding protein (A-FABP)-deficient mice have revealed that both basal and 
      isoproterenol-stimulated lipolysis were markedly reduced (Coe et al. 1999. J. 
      Lipid Res. 40: 967-972). Herein, we report the construction of transgenic mice 
      overexpressing the FABP5 gene encoding the epithelial fatty acid binding protein 
      (E-FABP) in adipocytes, thereby allowing evaluation of the effects on lipolysis 
      of increased FABP levels and of type specificity. In adipocytes from FABP5 
      transgenic mice, the total FABP protein level in the adipocyte was increased to 
      150% as compared to the wild type due to a 10-fold increase in the level of 
      E-FABP and an unanticipated 2-fold down-regulation of the A-FABP. There were no 
      significant differences in body weight, serum FFA, or fat pad mass between 
      wild-type and FABP5 transgenic mice. Importantly, both basal and 
      hormone-stimulated lipolysis increased in adipocytes from the FABP5 transgenic 
      animals. The molecular composition of the fatty acid pool from either the 
      intracellular compartment or that effluxed from the adipocyte was unaltered. 
      These results demonstrate that there is a positive relationship between lipolysis 
      and the total level of FABP but not between lipolysis and a specific FABP type.
FAU - Hertzel, Ann Vogel
AU  - Hertzel AV
AD  - Department of Biochemistry, Molecular Biology, and Biophysics, University of 
      Minnesota, 6-155 Jackson Hall, 321 Church St. SE, Minneapolis, MN 55455, USA.
FAU - Bennaars-Eiden, Assumpta
AU  - Bennaars-Eiden A
FAU - Bernlohr, David A
AU  - Bernlohr DA
LA  - eng
GR  - DK-49807/DK/NIDDK NIH HHS/United States
GR  - DK-53189/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
RN  - 0 (Carrier Proteins)
RN  - 0 (Fabp5 protein, mouse)
RN  - 0 (Fabp7 protein, mouse)
RN  - 0 (Fatty Acid-Binding Protein 7)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - L628TT009W (Isoproterenol)
SB  - IM
MH  - Adipocytes/*metabolism
MH  - Animals
MH  - Carrier Proteins/*genetics/*metabolism
MH  - Chromatography, Gas
MH  - Fatty Acid-Binding Protein 7
MH  - Fatty Acid-Binding Proteins
MH  - Isoproterenol/metabolism
MH  - *Lipid Metabolism
MH  - Lipolysis/*genetics/physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - *Neoplasm Proteins
MH  - *Nerve Tissue Proteins
MH  - Promoter Regions, Genetic
EDAT- 2002/11/28 04:00
MHDA- 2003/06/05 05:00
CRDT- 2002/11/28 04:00
PHST- 2002/11/28 04:00 [pubmed]
PHST- 2003/06/05 05:00 [medline]
PHST- 2002/11/28 04:00 [entrez]
AID - S0022-2275(20)32738-3 [pii]
AID - 10.1194/jlr.m200227-jlr200 [doi]
PST - ppublish
SO  - J Lipid Res. 2002 Dec;43(12):2105-11. doi: 10.1194/jlr.m200227-jlr200.