PMID- 12454993
OWN - NLM
STAT- MEDLINE
DCOM- 20030207
LR  - 20190906
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 455
IP  - 1
DP  - 2003 Jan 1
TI  - Brain-derived neurotrophic factor-, neurotrophin-3-, and tyrosine kinase 
      receptor-like immunoreactivity in lingual taste bud fields of mature hamster.
PG  - 11-24
AB  - The neurotrophins brain-derived neurotrophic factor (BDNF) and neurotrophin-3 
      (NT-3), as well as their respective tyrosine kinase (Trk) receptors, TrkB and 
      TrkC, influence peripheral target cell innervation, survival, and proliferation. 
      In the mature taste system the role of neurotrophins and their receptors is not 
      known. The mature hamster is an intriguing model because anterior lingual 
      fungiform, unlike posterior lingual foliate and circumvallate, taste buds survive 
      denervation. In light of this difference, we examined whether the degree of 
      neurotrophin- or neurotrophin receptor-like immunoreactivity (IR) normally 
      differs among lingual gemmal fields. In single- and double-labeled 
      immunofluorescent experiments, 3,209 taste bud sections (profiles) from 13 
      hamsters were examined for immunopositive gemmal cells or nerve fibers using 
      antibodies to BDNF and NT-3, their respective receptors TrkB and TrkC, and the 
      neural marker ubiquitin c-terminal hydrolase L-1 [protein gene product (PGP) 
      9.5]. In each gemmal field, more than 75% of taste bud profiles showed 
      immunopositivity to BDNF, NT-3, and TrkB. Across bud fields, BDNF-, TrkB-, and 
      BDNF/TrkB-like IR, as well as PGP 9.5 and PGP 9.5/BDNF-like IR in centrally 
      located, fungiform bud cells was greater (P < 0.0001 to P < 0.002) than in 
      circumvallate or foliate buds. Within bud fields, the number of BDNF-like, 
      labeled bud cells/bud profile was greater than that for NT-3-like IR in fungiform 
      (P < 0.0002) and foliate (P < 0.0001) buds. TrkC was immunonegative in gemmal 
      cells. The average density of TrkB- and TrkC-like fiber IR was more pronounced in 
      fungiform than posterior gemmal-bearing papillae. Thus, fungiform papillae, whose 
      taste buds are least affected by denervation, exhibit specific neurotrophin and 
      receptor enrichment.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Ganchrow, Donald
AU  - Ganchrow D
AD  - Department of Anatomy and Anthropology, Sackler Faculty of Medicine, Tel-Aviv 
      University, Ramat Aviv 69978, Tel-Aviv, Israel.
FAU - Ganchrow, Judith R
AU  - Ganchrow JR
FAU - Verdin-Alcazar, Mary
AU  - Verdin-Alcazar M
FAU - Whitehead, Mark C
AU  - Whitehead MC
LA  - eng
GR  - DC01901/DC/NIDCD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotrophin 3)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.10.1 (Receptor, trkC)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*analysis
MH  - Cricetinae
MH  - Immunohistochemistry
MH  - Male
MH  - Mesocricetus
MH  - Neurotrophin 3/*analysis
MH  - Receptor Protein-Tyrosine Kinases/*analysis
MH  - Receptor, trkB/analysis
MH  - Receptor, trkC/analysis
MH  - Taste Buds/*chemistry
MH  - *Tongue/innervation
EDAT- 2002/11/28 04:00
MHDA- 2003/02/08 04:00
CRDT- 2002/11/28 04:00
PHST- 2002/11/28 04:00 [pubmed]
PHST- 2003/02/08 04:00 [medline]
PHST- 2002/11/28 04:00 [entrez]
AID - 10.1002/cne.2162 [doi]
PST - ppublish
SO  - J Comp Neurol. 2003 Jan 1;455(1):11-24. doi: 10.1002/cne.2162.