PMID- 12456067
OWN - NLM
STAT- MEDLINE
DCOM- 20030110
LR  - 20191025
IS  - 0303-6995 (Print)
IS  - 0303-6995 (Linking)
IP  - 62
DP  - 2002
TI  - Neurotrophic factors and Alzheimer's disease: are we focusing on the wrong 
      molecule?
PG  - 241-52
AB  - Brain derived neurotrophic factor (BDNF) promotes cholinergic neuron function and 
      survival. In Alzheimer's disease, BDNF mRNA and protein are decreased in basal 
      forebrain cholinergic neuron target tissues such as cortex and hippocampus. Using 
      RT-PCR, we demonstrate that BDNF is synthesized in basal forebrain, supplying 
      cholinergic neurons with a local as well as a target-derived source of this 
      factor. BDNF mRNA levels are decreased 50% in nucleus basalis of Alzheimer 
      disease patients compared to controls. Thus, not only do the basal forebrain 
      cholinergic neurons have a reduced supply of target-derived BDNF, but also of 
      local BDNF. We also show by Western blotting that human CNS tissue contains both 
      proBDNF and mature BDNF protein. Moreover, we demonstrate a significant 
      (2.25-fold) deficit in proBDNF protein in Alzheimer's disease parietal cortex 
      compared to controls. Thus, reduced BDNF mRNA and protein levels in Alzheimer's 
      disease suggests that BDNF administration may be an effective therapeutic 
      strategy for this disorder.
FAU - Fahnestock, M
AU  - Fahnestock M
AD  - Department of Psychiatry and Behavioural Neurosciences, McMaster University, 
      Hamilton, Ont., Canada. fahnest@mcmaster.ca
FAU - Garzon, D
AU  - Garzon D
FAU - Holsinger, R M D
AU  - Holsinger RM
FAU - Michalski, B
AU  - Michalski B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - J Neural Transm Suppl
JT  - Journal of neural transmission. Supplementum
JID - 0425126
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Protein Precursors)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Alzheimer Disease/*pathology/*physiopathology
MH  - Basal Nucleus of Meynert/physiology
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/analysis/*genetics
MH  - Humans
MH  - Middle Aged
MH  - Parietal Lobe/chemistry/pathology
MH  - Protein Precursors/analysis/genetics
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2002/11/29 04:00
MHDA- 2003/01/11 04:00
CRDT- 2002/11/29 04:00
PHST- 2002/11/29 04:00 [pubmed]
PHST- 2003/01/11 04:00 [medline]
PHST- 2002/11/29 04:00 [entrez]
AID - 10.1007/978-3-7091-6139-5_22 [doi]
PST - ppublish
SO  - J Neural Transm Suppl. 2002;(62):241-52. doi: 10.1007/978-3-7091-6139-5_22.