PMID- 12456962
OWN - NLM
STAT- MEDLINE
DCOM- 20030512
LR  - 20061115
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 20
IP  - 6
DP  - 2002
TI  - Prevention of senile osteoporosis in SAMP6 mice by intrabone marrow injection of 
      allogeneic bone marrow cells.
PG  - 542-51
AB  - The SAMP6 mouse (a substrain of senescence-accelerated mice) spontaneously 
      develops osteoporosis early in life and is, therefore, a useful model for 
      examining the mechanisms underlying osteoporosis. We have recently established a 
      new bone marrow transplantation (BMT) method: the bone marrow cells (BMCs) of 
      normal allogeneic mice are directly injected into the bone marrow (BM) cavity of 
      irradiated (5.5 Gy x 2) recipients (IBM-BMT). Using IBM-BMT, we attempted to 
      prevent osteoporosis in SAMP6 mice. The hematolymphoid system was completely 
      reconstituted with donor-type cells after IBM-BMT. Thus-treated SAMP6 mice showed 
      marked increases in trabecular bones even at 12 months of age, and the bone 
      mineral density remained similar to that of normal B6 mice. In concordance with 
      these findings, urinary deoxypyridinoline also remained continuously low until 10 
      months of age, indicating that IBM-BMT was effective in the prevention of bone 
      absorption. In addition to the above, BM stromal cells in the treated SAMP6 mice 
      were replaced with donor stromal cells, and the message level of interleukin-11 
      (IL-11), which is produced by the BM stromal cells and is known as an important 
      factor in the regulation of bone remodeling, was restored to a level similar to 
      that observed in normal B6 mice. Furthermore, the message level of IL-6, which is 
      known to enhance osteoclastogenesis, was also restored to normal. These results 
      indicate that the BM microenvironment was normalized after IBM-BMT and that the 
      increased production of IL-11 and IL-6 ameliorated the imbalance between bone 
      absorption and formation, resulting in the prevention of osteoporosis in SAMP6 
      mice.
FAU - Ichioka, Naoya
AU  - Ichioka N
AD  - First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka, 
      Japan.
FAU - Inaba, Muneo
AU  - Inaba M
FAU - Kushida, Taketohi
AU  - Kushida T
FAU - Esumi, Takashi
AU  - Esumi T
FAU - Takahara, Kazuhiko
AU  - Takahara K
FAU - Inaba, Kayo
AU  - Inaba K
FAU - Ogawa, Ryokei
AU  - Ogawa R
FAU - Iida, Hirokazu
AU  - Iida H
FAU - Ikehara, Susumu
AU  - Ikehara S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Amino Acids)
RN  - 0 (Antigens, Surface)
RN  - 0 (Interleukin-11)
RN  - 0 (Interleukin-6)
RN  - 90032-33-0 (deoxypyridinoline)
SB  - IM
MH  - Amino Acids/urine
MH  - Animals
MH  - Antigens, Surface/analysis
MH  - Bone Density
MH  - *Bone Marrow Transplantation
MH  - Cells, Cultured
MH  - Female
MH  - Flow Cytometry
MH  - Gene Expression
MH  - Immune System/physiology
MH  - Infusions, Intraosseous
MH  - Interleukin-11/analysis/genetics
MH  - Interleukin-6/analysis/genetics
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Mutant Strains
MH  - Osteoporosis/genetics/pathology/*prevention & control
MH  - Stromal Cells/chemistry/cytology/*transplantation
MH  - Transplantation, Homologous
EDAT- 2002/11/29 04:00
MHDA- 2003/05/13 05:00
CRDT- 2002/11/29 04:00
PHST- 2002/11/29 04:00 [pubmed]
PHST- 2003/05/13 05:00 [medline]
PHST- 2002/11/29 04:00 [entrez]
AID - 10.1634/stemcells.20-6-542 [doi]
PST - ppublish
SO  - Stem Cells. 2002;20(6):542-51. doi: 10.1634/stemcells.20-6-542.