PMID- 12457940
OWN - NLM
STAT- MEDLINE
DCOM- 20030408
LR  - 20190916
IS  - 0741-8329 (Print)
IS  - 0741-8329 (Linking)
VI  - 28
IP  - 2
DP  - 2002 Oct
TI  - Sex differences in the modulation by ethanol of lung chemotaxis.
PG  - 95-102
AB  - We studied the influence of sex on the modulation by acute ethanol intoxication 
      of lung polymorphonuclear leukocyte (PMN) recruitment, production of chemotactic 
      factors, and nuclear factor kappa-B (NF-kappa B) activation in alveolar 
      macrophages (AMs) of rats receiving an intrapulmonary challenge with endotoxin 
      (ET). Male and female Charles River rats were given an intratracheal ET challenge 
      [100 micro g in phosphate-buffered saline (PBS)], followed by an intravenous 
      infusion of ethanol or saline for 2.5 h. At that time, bronchoalveolar lavage 
      (BAL) fluid was obtained, and AMs and recruited PMNs were isolated. Acute ethanol 
      treatment [primed 2.5-h intravenous infusion of ethanol, priming dose of 0.87 ml 
      per 100 grams of body weight of 20% (vol./vol.) ethanol, followed by a continuous 
      infusion of 20% ethanol at 0.15 ml per 100 grams of body weight per hour] 
      suppressed ET-induced lung PMN recruitment equally in female and male rats. 
      However, cytokine-induced neutrophil chemoattractant (CINC) and macrophage 
      inflammatory protein-2 (MIP-2) in BAL fluid were suppressed only in female rats. 
      Polymorphonuclear leukocytes of untreated female rats responded to MIP-2 and 
      formyl-methionyl-leucyl-phenylalanine (fMLP) with lower chemotactic activity than 
      did PMNs of male rats. Activation of NF-kappa B in AMs of female rats treated 
      with ET or with ET plus ethanol was less than that in male rats, supporting the 
      suggestion of transcriptional regulation of chemoattractant production, leading 
      to reduced PMN recruitment. Because excessive PMN recruitment with subsequent 
      release of granular contents is associated with tissue damage, these results 
      indicate a potential protective mechanism against pulmonary damage in female 
      rats.
FAU - Spitzer, Judy A
AU  - Spitzer JA
AD  - Department of Physiology and Alcohol Research Center, Louisiana State University 
      Health Sciences Center, 1901 Perdido Street, New Orleans, LA 70112, USA. 
      jspitzl@lsumc.edu
FAU - Zhang, Ping
AU  - Zhang P
FAU - Bagby, Gregory J
AU  - Bagby GJ
FAU - Stouwe, Curtis Vande
AU  - Stouwe CV
FAU - Nelson, Steve
AU  - Nelson S
LA  - eng
GR  - AA09803/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Alcohol
JT  - Alcohol (Fayetteville, N.Y.)
JID - 8502311
RN  - 0 (NF-kappa B)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Animals
MH  - Chemotaxis, Leukocyte/*drug effects/immunology
MH  - Ethanol/*pharmacology
MH  - Female
MH  - Lung/cytology/*drug effects/immunology
MH  - Macrophages, Alveolar/cytology/drug effects/immunology
MH  - Male
MH  - NF-kappa B/immunology/metabolism
MH  - Neutrophils/cytology/*drug effects/immunology
MH  - Rats
MH  - *Sex Characteristics
EDAT- 2002/11/30 04:00
MHDA- 2003/04/09 05:00
CRDT- 2002/11/30 04:00
PHST- 2002/11/30 04:00 [pubmed]
PHST- 2003/04/09 05:00 [medline]
PHST- 2002/11/30 04:00 [entrez]
AID - S0741832902002719 [pii]
AID - 10.1016/s0741-8329(02)00271-9 [doi]
PST - ppublish
SO  - Alcohol. 2002 Oct;28(2):95-102. doi: 10.1016/s0741-8329(02)00271-9.