PMID- 12458164
OWN - NLM
STAT- MEDLINE
DCOM- 20030326
LR  - 20190901
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 91
IP  - 2-3
DP  - 2003 Feb 2
TI  - Entry and survival of Salmonella enterica serotype Enteritidis PT4 in chicken 
      macrophage and lymphocyte cell lines.
PG  - 147-55
AB  - Various leukocytes are involved in the reaction to counter Salmonella infection 
      in chicken. The various leukocyte types react differently after an infection, 
      since some clear the infection while others may cause dissemination of Salmonella 
      throughout the chicken. Therefore, we investigated in vitro the entry and 
      survival of Salmonella enterica serotype Enteritidis in chicken cell lines of 
      various cell types, including two macrophage cell lines, HD11 and MQ-NCSU (NCSU), 
      two B-cell lines LSCC-1104-X5 (1104) and LSCC-RP9 (RP9), and a T-cell line 
      MDCC-MSB-1 (MSB-1). The macrophages were able to internalize high numbers of S. 
      Enteritidis. In contrast and as expected, cells of the T-cell line MSB-1 and the 
      B-cell line RP9 internalized bacteria at a much lower level. After S. Enteritidis 
      entered the macrophages, the number of intracellular S. Enteritidis decreased 
      over time, so that after 48h no more than 20% of the bacteria, which had entered, 
      survived intracellularly. In contrast to macrophages, the number of S. 
      Enteritidis in cells of the T-cell line MSB-1 and the B-cell line RP9 increased 
      rapidly within 12h post-inoculation. Thereafter the number of intracellular S. 
      Enteritidis decreased only slowly. In conclusion, all three different cell types 
      were able to control and to start clearing S. Enteritidis, although macrophages 
      were far more effective compared to T- and B-cells. However, none of the cell 
      lines were able to clear S. Enteritidis fully within 48h. These results suggest 
      that the three cell types play an important but different role in the 
      dissemination and elimination of S. Enteritidis throughout the animal.
FAU - Kramer, J
AU  - Kramer J
AD  - Institute of Animal Science and Health, ID-Lelystad B.V., 8200 AB, Lelystad, The 
      Netherlands. j.kramer@id.wag-ur.nl
FAU - Visscher, A H
AU  - Visscher AH
FAU - Wagenaar, J A
AU  - Wagenaar JA
FAU - Jeurissen, S H M
AU  - Jeurissen SH
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Coloring Agents)
RN  - I2ZWO3LS3M (Trypan Blue)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/immunology/*microbiology
MH  - *Chickens
MH  - Coloring Agents/chemistry
MH  - Kinetics
MH  - Macrophages/immunology/*microbiology
MH  - Poultry Diseases/immunology/*microbiology
MH  - Salmonella Infections, Animal/immunology/*microbiology
MH  - Salmonella enteritidis/*growth & development/immunology
MH  - T-Lymphocytes/immunology/*microbiology
MH  - Trypan Blue/chemistry
EDAT- 2002/11/30 04:00
MHDA- 2003/03/27 05:00
CRDT- 2002/11/30 04:00
PHST- 2002/11/30 04:00 [pubmed]
PHST- 2003/03/27 05:00 [medline]
PHST- 2002/11/30 04:00 [entrez]
AID - S0378113502003048 [pii]
AID - 10.1016/s0378-1135(02)00304-8 [doi]
PST - ppublish
SO  - Vet Microbiol. 2003 Feb 2;91(2-3):147-55. doi: 10.1016/s0378-1135(02)00304-8.