PMID- 12458327
OWN - NLM
STAT- MEDLINE
DCOM- 20030326
LR  - 20061115
IS  - 0028-2685 (Print)
IS  - 0028-2685 (Linking)
VI  - 49
IP  - 5
DP  - 2002
TI  - Detection of 13q abnormalities in multiple myeloma using immunomagnetically 
      selected plasma cells.
PG  - 300-6
AB  - Accurate prognostic evaluation of patients with multiple myeloma (MM) is required 
      for their stratification for more adequate therapy. Chromosomal G-banding and 
      interphase fluorescence in situ hybridization (FISH) on cell-nonspecific samples 
      and on myeloma cells selected by magnetic-activated cell separation (MACS) were 
      used to study 13 samples from 12 multiple myeloma (MM) patients. Bone marrow (BM) 
      samples were analysed using three approaches. Standard mitotic samples were 
      prepared and analysed after G-banding. Interphase FISH was performed to detect 
      the 13q14 deletion in unselected BM cells. In parallel, myeloma cells were 
      selected from the BM using the CD138-specific antibody. The high-purity myeloma 
      cell suspension was then analysed by interphase FISH for the 13q14 deletion. 
      Magnetic separation yielded enriched myeloma cell suspensions with the mean 
      viability of 98.0% (range: 97.0%-99.0%), and the purity of 97.6% (range: 
      87.2%-99.2%) as detected morphologically, and 85.2% (range: 44.8%-98.4%) as 
      detected by immunophenotyping for CD138+ cells. Interphase FISH revealed the 
      13q14.3 deletion in 5 of 13 (38.5%) of cell-nonspecific samples and in 9 of 13 
      (69.2%) of enriched myeloma cell suspensions. In conclusion, interphase FISH on 
      immunomagnetically selected MM cells increases the detection of the 13q14 
      deletion in BM samples from the patients with MM.
FAU - Fiserova, A
AU  - Fiserova A
AD  - Department of Clinical Hematology; Masaryk University Hospital, Brno, 63900 Czech 
      Republic. tbuchler@fnbrno.cz
FAU - Hajek, R
AU  - Hajek R
FAU - Holubova, V
AU  - Holubova V
FAU - Buchler, T
AU  - Buchler T
FAU - Sobotka, J
AU  - Sobotka J
FAU - Kovarova, R
AU  - Kovarova R
FAU - Musilova, R
AU  - Musilova R
FAU - Bourkova, L
AU  - Bourkova L
FAU - Bulikova, A
AU  - Bulikova A
FAU - Mareschova, I
AU  - Mareschova I
FAU - Janackova, Z
AU  - Janackova Z
FAU - Vanova, P
AU  - Vanova P
FAU - Kuglik, P
AU  - Kuglik P
FAU - Vorlicek, J
AU  - Vorlicek J
FAU - Penka, M
AU  - Penka M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Slovakia
TA  - Neoplasma
JT  - Neoplasma
JID - 0377266
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Proteoglycans)
RN  - 0 (SDC1 protein, human)
RN  - 0 (Syndecan-1)
RN  - 0 (Syndecans)
SB  - IM
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - *Chromosomes, Human, Pair 13
MH  - Gene Deletion
MH  - Humans
MH  - *Immunomagnetic Separation
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Magnetics
MH  - Membrane Glycoproteins/biosynthesis
MH  - Mitosis
MH  - Multiple Myeloma/blood/*genetics
MH  - Proteoglycans/biosynthesis
MH  - Syndecan-1
MH  - Syndecans
EDAT- 2002/11/30 04:00
MHDA- 2003/03/27 05:00
CRDT- 2002/11/30 04:00
PHST- 2002/11/30 04:00 [pubmed]
PHST- 2003/03/27 05:00 [medline]
PHST- 2002/11/30 04:00 [entrez]
PST - ppublish
SO  - Neoplasma. 2002;49(5):300-6.