PMID- 12459032
OWN - NLM
STAT- MEDLINE
DCOM- 20031015
LR  - 20191025
IS  - 0952-5041 (Print)
IS  - 0952-5041 (Linking)
VI  - 29
IP  - 3
DP  - 2002 Dec
TI  - The multiple endocrine neoplasia type 1 gene product, menin, inhibits the human 
      prolactin promoter activity.
PG  - 297-304
AB  - Menin is a protein encoded by the gene mutated in multiple endocrine neoplasia 
      type 1 (MEN1) characterized by multiple endocrine tumors of the parathyroid 
      glands, pancreatic islets and the anterior pituitary, especially prolactinoma. In 
      this study, we examined the effects of menin on human prolactin (hPRL) 
      expression. In rat pituitary GH3 cells stably expressing menin, both PRL gene 
      expression/secretion and thymidine incorporation into DNA were inhibited as 
      compared with mock-transfected cells. The transcriptional activity of PRL 
      promoter in GH3 cells co-transfected with menin was significantly decreased. A 
      deletion mutation (569 delC), which we identified in a Japanese MEN1 family, was 
      introduced into menin. When GH3 cells were transfected with a mutant menin 
      expression vector, inhibition of hPRL promoter activity was partially reversed. 
      These observations suggest that menin inhibits hPRL promoter activity and cell 
      proliferation, raising the possibility that menin might play an important role in 
      the tumorigenesis of prolactinoma.
FAU - Namihira, H
AU  - Namihira H
AD  - First Department of Internal Medicine, Kagawa Medical University, 1750-1 Ikenobe 
      Miki-Cho, Kita-gun, Kagawa, Japan.
FAU - Sato, M
AU  - Sato M
FAU - Murao, K
AU  - Murao K
FAU - Cao, W M
AU  - Cao WM
FAU - Matsubara, S
AU  - Matsubara S
FAU - Imachi, H
AU  - Imachi H
FAU - Niimi, M
AU  - Niimi M
FAU - Dobashi, H
AU  - Dobashi H
FAU - Wong, N C W
AU  - Wong NC
FAU - Ishida, T
AU  - Ishida T
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Mol Endocrinol
JT  - Journal of molecular endocrinology
JID - 8902617
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 9002-62-4 (Prolactin)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Division
MH  - Cell Line
MH  - *Down-Regulation
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Multiple Endocrine Neoplasia Type 1/genetics
MH  - Neoplasm Proteins/genetics/*metabolism
MH  - Prolactin/*genetics/metabolism
MH  - Promoter Regions, Genetic/*genetics
MH  - *Proto-Oncogene Proteins
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Transcription, Genetic
MH  - Transfection
EDAT- 2002/12/03 04:00
MHDA- 2003/10/16 05:00
CRDT- 2002/12/03 04:00
PHST- 2002/12/03 04:00 [pubmed]
PHST- 2003/10/16 05:00 [medline]
PHST- 2002/12/03 04:00 [entrez]
AID - 10.1677/jme.0.0290297 [doi]
PST - ppublish
SO  - J Mol Endocrinol. 2002 Dec;29(3):297-304. doi: 10.1677/jme.0.0290297.