PMID- 12459248
OWN - NLM
STAT- MEDLINE
DCOM- 20030409
LR  - 20191106
IS  - 0167-7012 (Print)
IS  - 0167-7012 (Linking)
VI  - 52
IP  - 2
DP  - 2003 Feb
TI  - Development of a fluorescence in situ hybridization method for cheese using a 16S 
      rRNA probe.
PG  - 267-71
AB  - A 16S rRNA fluorescence in situ hybridization (FISH) method for cheese was 
      developed to allow detection in situ of microorganisms within the dairy matrix. 
      An embedding procedure using a plastic resin was applied to Stilton cheese, 
      providing intact embedded cheese sections withstanding the hybridization 
      reaction. The use of a fluorescein-labelled 16S rRNA Domain Bacteria probe 
      allowed observation of large colonies of microbial cells homogeneously 
      distributed in the cheese matrix. FISH experiments performed on cheese 
      suspensions provided images of the different microbial morphotypes occurring. The 
      technique has great potential to study the spatial distribution of microbial 
      populations in situ in foods, especially where the matrix is too fragile to allow 
      manipulation of cryosections.
CI  - Copyright 2003 Elsevier Science B.V.
FAU - Ercolini, Danilo
AU  - Ercolini D
AD  - Division of Food Sciences, School of Biosciences, University of Nottingham, 
      Sutton Bonington Campus, Loughborough, Leicestershire LE125RD, UK.
FAU - Hill, Philip J
AU  - Hill PJ
FAU - Dodd, Christine E R
AU  - Dodd CE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (RNA Probes)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Cheese/*microbiology
MH  - Dairy Products
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *RNA Probes
MH  - RNA, Bacterial/*analysis
MH  - RNA, Ribosomal, 16S/*analysis/genetics
EDAT- 2002/12/03 04:00
MHDA- 2003/04/10 05:00
CRDT- 2002/12/03 04:00
PHST- 2002/12/03 04:00 [pubmed]
PHST- 2003/04/10 05:00 [medline]
PHST- 2002/12/03 04:00 [entrez]
AID - S0167701202001628 [pii]
AID - 10.1016/s0167-7012(02)00162-8 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2003 Feb;52(2):267-71. doi: 10.1016/s0167-7012(02)00162-8.