PMID- 12459653
OWN - NLM
STAT- MEDLINE
DCOM- 20030627
LR  - 20191025
IS  - 0960-8931 (Print)
IS  - 0960-8931 (Linking)
VI  - 12
IP  - 6
DP  - 2002 Dec
TI  - Urinary excretion of epidermal-type fatty acid-binding protein and S100A7 protein 
      in patients with cutaneous melanoma.
PG  - 627-31
AB  - Epidermal-type fatty acid-binding protein (E-FABP), a protein related to the 
      intracellular trafficking of fatty acids, is expressed in melanocytic tumours but 
      not in normal human melanocytes. E-FABP interacts with S100A7. The presence of 
      these two proteins was investigated in the urine of patients with cutaneous 
      melanoma or other types of cancer, and healthy controls. The first voided morning 
      urine samples of 31 patients with melanoma, 73 patients with other types of 
      cancer and 17 healthy controls were concentrated and submitted to sodium dodecyl 
      sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) immunoblotting for protein 
      detection. In the healthy controls, the incidences of urinary detection of these 
      proteins were higher in females than in males, being 50% (five out of 10) versus 
      0% (zero out of seven) for E-FABP ( < 0.05), and 70% (seven out of 10) versus 0% 
      (zero out of seven) for S100A7 ( < 0.05). Both proteins were detected in the 
      urine of patients with melanoma. The incidence of S100A7 was higher in the urine 
      of patients with melanoma (77%, 24 out of 31) compared with healthy controls 
      (41%, seven out of 17) and patients with other types of cancer (53%, 39 out of 
      73) ( < 0.03). In contrast, the incidence of E-FABP was the same among the 
      melanoma group (39%, 12 out of 31), healthy controls (29%, five out of 17) and 
      patients with other types of cancer (23%, 17 out of 73). Surprisingly, E-FABP was 
      always detected in the urine of females with stage I/II or III melanoma, but was 
      no longer detectable in the urine of patients with stage IV melanoma. Urinary 
      S100A7 may have some specificity to the host response to melanoma since its 
      incidence was not increased in other cancers. The lack of E-FABP detection in the 
      urine of patients with distant metastases suggests an inverse relationship 
      between E-FABP release and the spread of melanoma.
FAU - Brouard, M C
AU  - Brouard MC
AD  - Department of Dermatology, University Hospital, Geneva, Switzerland. 
      mcvpb@hotmail.com
FAU - Saurat, J-H
AU  - Saurat JH
FAU - Ghanem, G
AU  - Ghanem G
FAU - Siegenthaler, G
AU  - Siegenthaler G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Melanoma Res
JT  - Melanoma research
JID - 9109623
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (FABP5 protein, human)
RN  - 0 (FABP7 protein, human)
RN  - 0 (Fatty Acid-Binding Protein 7)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (S100 Calcium Binding Protein A7)
RN  - 0 (S100 Proteins)
RN  - 0 (S100A7 protein, human)
RN  - 0 (Tumor Suppressor Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/*urine
MH  - Calcium-Binding Proteins/*urine
MH  - Carrier Proteins/*urine
MH  - Fatty Acid-Binding Protein 7
MH  - Fatty Acid-Binding Proteins
MH  - Female
MH  - Humans
MH  - Male
MH  - Melanoma/*urine
MH  - Middle Aged
MH  - *Neoplasm Proteins
MH  - Reference Values
MH  - S100 Calcium Binding Protein A7
MH  - S100 Proteins
MH  - Sex Factors
MH  - Skin Neoplasms/*urine
MH  - *Tumor Suppressor Proteins
EDAT- 2002/12/03 04:00
MHDA- 2003/06/28 05:00
CRDT- 2002/12/03 04:00
PHST- 2002/12/03 04:00 [pubmed]
PHST- 2003/06/28 05:00 [medline]
PHST- 2002/12/03 04:00 [entrez]
AID - 10.1097/00008390-200212000-00013 [doi]
PST - ppublish
SO  - Melanoma Res. 2002 Dec;12(6):627-31. doi: 10.1097/00008390-200212000-00013.