PMID- 12460423
OWN - NLM
STAT- MEDLINE
DCOM- 20030213
LR  - 20191210
IS  - 0266-8254 (Print)
IS  - 0266-8254 (Linking)
VI  - 35
IP  - 6
DP  - 2002
TI  - Laccase stabilization by covalent binding immobilization on activated polyvinyl 
      alcohol carrier.
PG  - 451-6
AB  - AIMS: Attempts were made to immobilize laccase from Panus conchatus. METHODS AND 
      RESULTS: The laccase was immobilized on carboxylated polyvinyl alcohol (PVA) 
      activated by N-hydroxysuccinimide (N-HSI) in aqueous solution at different pHs, 
      temperatures, and with different reaction times. An optimum condition for laccase 
      immobilization is at pH 3.2, 40 degrees C and 12 h, respectively. Immobilization 
      of laccase increased optimal pH for reaction with 2, 2'-azinobis 
      (3-ethylbenzthiazoline-6-solfonate) (ABTS) and pH stability. Immobilized laccase 
      proved to be reacted consecutively 17 times with only a 50% decrease on activity 
      and be used in removal of 2,4,6-trichlorophenol (TCP). CONCLUSIONS: It was 
      possible to immobilize the laccase on carboxylated polyvinyl alcohol by 
      activation with N-hydroxysuccinimide in HAc-NaAc buffer. The immobilized laccase 
      is both stable and reusable. SIGNIFICANCE IMPACT OF THE STUDY: The results 
      indicate that this immobilized laccase can be used in the removal of poisonous 
      effluent from pulp bleaching mills.
FAU - Yinghui, Diao
AU  - Yinghui D
AD  - Laboratory of Cellulose and Lignocellulosics Chemistry, Guangzhou Institute of 
      Chemistry, Chinese Academy of Sciences, Guanzhou, PR, China.
FAU - Qiuling, Wang
AU  - Qiuling W
FAU - Shiyu, Fu
AU  - Shiyu F
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Lett Appl Microbiol
JT  - Letters in applied microbiology
JID - 8510094
RN  - 0 (Chlorophenols)
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Industrial Waste)
RN  - 9002-89-5 (Polyvinyl Alcohol)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.10.3.2 (Laccase)
RN  - MHS8C5BAUZ (2,4,6-trichlorophenol)
SB  - IM
MH  - Basidiomycota/*enzymology
MH  - Chlorophenols/metabolism
MH  - Enzyme Stability
MH  - *Enzymes, Immobilized
MH  - Industrial Waste
MH  - Laccase
MH  - Oxidoreductases/*chemistry/*metabolism
MH  - Paper
MH  - Polyvinyl Alcohol/*chemistry
MH  - Waste Disposal, Fluid/methods
EDAT- 2002/12/04 04:00
MHDA- 2003/02/14 04:00
CRDT- 2002/12/04 04:00
PHST- 2002/12/04 04:00 [pubmed]
PHST- 2003/02/14 04:00 [medline]
PHST- 2002/12/04 04:00 [entrez]
AID - 1196 [pii]
AID - 10.1046/j.1472-765x.2002.01196.x [doi]
PST - ppublish
SO  - Lett Appl Microbiol. 2002;35(6):451-6. doi: 10.1046/j.1472-765x.2002.01196.x.