PMID- 12461648
OWN - NLM
STAT- MEDLINE
DCOM- 20030520
LR  - 20071114
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 13
IP  - 11
DP  - 2002 Nov
TI  - Genetic mapping of an insertional hydrocephalus-inducing mutation allelic to hy3.
PG  - 625-32
AB  - The transgenic mouse line OVE459 carries a transgene-induced insertional mutation 
      resulting in autosomal recessive congenital hydrocephalus. Homozygous transgenic 
      animals experience ventricular dilation with perinatal onset and are noticeably 
      smaller than hemizygous or non-transgenic littermates within a few days after 
      birth. Fluorescence in situ hybridization (FISH) revealed that the transgene 
      inserted in a single locus on mouse Chromosome (chr) 8, region D2-E1. Genetic 
      crosses between hemizygous OVE459 mice and mice heterozygous for the spontaneous 
      mutation hydrocephalus-3 (hy3) produced hydrocephalic offspring with a frequency 
      of 22%, demonstrating that these two mutations are allelic. A genomic library was 
      made by using DNA from homozygous OVE459 mice, and genomic DNA flanking the 
      transgene insertion site was isolated and sequenced. A PCR polymorphism between 
      C57BL/6 DNA and Mus spretus was used to map the location of the transgene insert 
      to 1.06 cM +/- 0.75 proximal to D8Mit152 by using the Jackson Laboratory 
      Backcross DNA Panel Mapping Resource. Furthermore, sequence analysis from a mouse 
      bacterial artificial chromosome (BAC) clone, positive for unique markers on both 
      sides of the transgene insertion site, demonstrated that the genomic DNAs 
      flanking each side of the transgene insertion are physically separated by 
      approximately 51 kb on the wild-type mouse chromosome.
FAU - Robinson, Michael L
AU  - Robinson ML
AD  - Division of Molecular and Human Genetics, Dept. of Pediatrics, Children's 
      Research Institute, The Ohio State University, 700 Children's Drive, Rm. W492, 
      Columbus, OH 43205, USA. robinsom@pediatrics.ohio-state.edu
FAU - Allen, Carl E
AU  - Allen CE
FAU - Davy, Brian E
AU  - Davy BE
FAU - Durfee, William J
AU  - Durfee WJ
FAU - Elder, Frederick F
AU  - Elder FF
FAU - Elliott, Christopher S
AU  - Elliott CS
FAU - Harrison, Wilbur R
AU  - Harrison WR
LA  - eng
SI  - GENBANK/AC069308
GR  - HD 34615/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
SB  - IM
MH  - Animals
MH  - Female
MH  - Humans
MH  - Hydrocephalus/*genetics
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - *Physical Chromosome Mapping
EDAT- 2002/12/04 04:00
MHDA- 2003/05/21 05:00
CRDT- 2002/12/04 04:00
PHST- 2002/05/28 00:00 [received]
PHST- 2002/08/21 00:00 [accepted]
PHST- 2002/12/04 04:00 [pubmed]
PHST- 2003/05/21 05:00 [medline]
PHST- 2002/12/04 04:00 [entrez]
AID - 10.1007/s00335-002-2201-8 [doi]
PST - ppublish
SO  - Mamm Genome. 2002 Nov;13(11):625-32. doi: 10.1007/s00335-002-2201-8.