PMID- 12461750
OWN - NLM
STAT- MEDLINE
DCOM- 20030117
LR  - 20061115
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 36
IP  - 1
DP  - 2003 Jan
TI  - Deletion mapping on the long arm of chromosome 7 in splenic lymphoma with villous 
      lymphocytes.
PG  - 57-69
AB  - Splenic lymphoma with villous lymphocytes (SLVL) is a low-grade 
      lymphoproliferative disorder characterized by splenomegaly and circulating 
      villous lymphocytes in the peripheral blood. It is considered to be the leukemic 
      form of splenic marginal zone lymphoma (SMZL). The genetic basis of this lymphoma 
      type remains unknown. Conventional cytogenetic studies have identified frequent 
      structural abnormalities of chromosome 7, in the form of translocations, mainly 
      unbalanced, and 7q deletions. In this current study, we undertook deletion 
      mapping of the long arm of chromosome 7 in a series of cases with SLVL. Metaphase 
      fluorescence in situ hybridization (FISH) was used in the first instance, 
      followed by a study of loss of heterozygosity (LOH). The common area of deletion 
      identified by FISH spanned from the YAC clone HSC7E1289 (mapping to 7q32.1) to in 
      between YACs HSC7E195 and HSC7E648 (7q32-3). By application of 50 microsatellite 
      markers mapping to the FISH-CDR and to areas of deletion reported in other 
      studies, four distinct hotspot loci were identified, with abnormalities present 
      in 29-55% cases. In three of them, both LOH and biallelic deletions were found. 
      The LOH in the majority of patients was noncontiguous. The presence of a high 
      incidence of abnormalities in the established hotspot areas and in particular the 
      finding of biallelic deletions is indicative of the existence of genes important 
      for the pathogenesis of SLVL in these areas.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Gruszka-Westwood, Alicja M
AU  - Gruszka-Westwood AM
AD  - Academic Department of Haematology and Cytogenetics, Institute of Cancer 
      Research/Royal Marsden NHS Trust, London, United Kingdom.
FAU - Hamoudi, Rifat
AU  - Hamoudi R
FAU - Osborne, Lucy
AU  - Osborne L
FAU - Matutes, Estella
AU  - Matutes E
FAU - Catovsky, Daniel
AU  - Catovsky D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Genetic Markers)
SB  - IM
EIN - Genes Chromosomes Cancer. 2004 Feb;39(2):170
MH  - Allelic Imbalance/genetics
MH  - B-Lymphocytes/*pathology
MH  - *Chromosome Deletion
MH  - Chromosome Mapping/*methods
MH  - Chromosomes, Artificial, Yeast/genetics
MH  - Chromosomes, Human, Pair 7/*genetics
MH  - DNA Probes/genetics
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - Genetic Markers/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Karyotyping/methods
MH  - Loss of Heterozygosity/genetics
MH  - Lymphoma, B-Cell/*genetics
MH  - Male
MH  - Splenic Neoplasms/*genetics
EDAT- 2002/12/04 04:00
MHDA- 2003/01/18 04:00
CRDT- 2002/12/04 04:00
PHST- 2002/12/04 04:00 [pubmed]
PHST- 2003/01/18 04:00 [medline]
PHST- 2002/12/04 04:00 [entrez]
AID - 10.1002/gcc.10142 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2003 Jan;36(1):57-69. doi: 10.1002/gcc.10142.