PMID- 12462419
OWN - NLM
STAT- MEDLINE
DCOM- 20030127
LR  - 20190822
IS  - 0364-3190 (Print)
IS  - 0364-3190 (Linking)
VI  - 27
IP  - 10
DP  - 2002 Oct
TI  - Expression profiling to understand actions of NMDA/glutamate receptor antagonists 
      in rat brain.
PG  - 1209-20
AB  - Agents acting as noncompetitive N-methyl-D-aspartate (NMDA)/glutamate receptor 
      antagonists induce the expression of several genes in limbic cortical regions, 
      such as the cingulate, retrosplenial, and entorhinal cortices. These include 
      important regulatory genes such as the neurotrophin brain-derived neurotrophic 
      factor (BDNF), its receptor trkB, and c-fos. We applied expression profiling 
      methods to find genes coregulated with BDNF following treatment with the 
      prototypical NMDA/glutamate receptor antagonist MK-801. Expression profiling 
      provides a useful technique for describing the molecular and transcriptional 
      level events that follow various processes. We illustrate the utility of 
      microarrays to find novel ESTs regulated by MK-801. We also used expression 
      profiling with microarrays to characterize the levels of transcription factor 
      cAMP response element modulator (CREM) and inducible cAMP early repressor (ICER) 
      isoforms that are induced by MK-801. These factors may act as the eventual 
      repressors for BDNF expression via competition and heterodimerization with 
      phosphorylated CREB, a transcription factor important for BDNF expression. 
      Finally, we find and confirm the regulation of Erp29, RTNI, and an ABC 
      transporter by antagonism of NMDA/glutamate receptors as potential stress related 
      molecules in brain. The emerging picture generated by using these expression 
      profiling approaches, identifies several of what likely will be many molecules 
      that take part in the complex events that occur during BDNF signaling mediated by 
      blockade of NMDA/ glutamate receptors.
FAU - Toronen, Petri
AU  - Toronen P
AD  - Laboratory of Molecular Pharmacology, Neurobiology Department, A. I. Virtanen 
      Institute, Kuopio University, Finland.
FAU - Storvik, Marcus
AU  - Storvik M
FAU - Linden, Anni-Maija
AU  - Linden AM
FAU - Kontkane, Outi
AU  - Kontkane O
FAU - Marvanova, Marketa
AU  - Marvanova M
FAU - Lakso, Merja
AU  - Lakso M
FAU - Castren, Eero
AU  - Castren E
FAU - Wong, Garry
AU  - Wong G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Receptors, Glutamate)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
SB  - IM
MH  - Animals
MH  - Brain/*metabolism/physiology
MH  - Excitatory Amino Acid Antagonists/*pharmacology
MH  - *Gene Expression Profiling
MH  - In Situ Hybridization
MH  - Male
MH  - Oligonucleotide Array Sequence Analysis
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Glutamate/*drug effects
MH  - Receptors, N-Methyl-D-Aspartate/*antagonists & inhibitors
EDAT- 2002/12/05 04:00
MHDA- 2003/01/28 04:00
CRDT- 2002/12/05 04:00
PHST- 2002/12/05 04:00 [pubmed]
PHST- 2003/01/28 04:00 [medline]
PHST- 2002/12/05 04:00 [entrez]
AID - 10.1023/a:1020985611667 [doi]
PST - ppublish
SO  - Neurochem Res. 2002 Oct;27(10):1209-20. doi: 10.1023/a:1020985611667.