PMID- 12464022
OWN - NLM
STAT- MEDLINE
DCOM- 20030610
LR  - 20231213
IS  - 0268-9146 (Print)
IS  - 0268-9146 (Linking)
VI  - 33
IP  - 6
DP  - 2002 Dec
TI  - Mapping of equine potassium chloride co-transporter (SLC12A4) and amino acid 
      transporter (SLC7A10) and preliminary studies on associations between SNPs from 
      SLC12A4, SLC7A10 and SLC7A9 and osmotic fragility of erythrocytes.
PG  - 455-9
AB  - Consensus DNA sequences from human, mouse and/or rat were used to design 
      oligonucleotide primers for equine homologues of exons 16, 17 and 20-23 of 
      potassium chloride co-transporter (SLC12A4) and exons 10, 11 and 3, 4, 
      respectively, for two amino acid transporters (SLC7A10 and SLC7A9). DNA sequences 
      of the PCR products showed high sequence identity to these regions. Equine BAC 
      clones were obtained for SLC12A4 and SLC7A10 and mapped to equine chromosomes 
      ECA3p13 and ECA10p15, respectively, by fluorescence in situ hybridization (FISH). 
      Several single nucleotide polymorphisms (SNP) were found. Substitutions of A/G 
      were found within exon 17 of SLC12A4, within intron 11 of SLC7A10 and within 
      intron 3 of SLC7A9. The SNP associated with SLC7A10 and SLC7A9 were sufficiently 
      polymorphic to investigate associations with erythrocyte fragility among a group 
      of 20 thoroughbred horses. A non-parametric rank-sum test showed a weak 
      association between erythrocyte fragility and the SNP associated with SLC7A10 (P 
      < 0.05).
FAU - Hanzawa, K
AU  - Hanzawa K
AD  - Department of Veterinary Science, MH Gluck Equine Research Center, University of 
      Kentucky, Lexington, KY, USA. khanzawa@nodai.ac.jp
FAU - Lear, T L
AU  - Lear TL
FAU - Piumi, F
AU  - Piumi F
FAU - Bailey, E
AU  - Bailey E
LA  - eng
PT  - Journal Article
PL  - England
TA  - Anim Genet
JT  - Animal genetics
JID - 8605704
RN  - 0 (Amino Acid Transport System y+)
RN  - 0 (Amino Acids)
RN  - 0 (Symporters)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acid Transport System y+/*genetics
MH  - Amino Acids/metabolism
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping/veterinary
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - DNA/chemistry/genetics
MH  - Erythrocyte Deformability/*genetics
MH  - Horses/blood/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Osmotic Fragility
MH  - Polymerase Chain Reaction/veterinary
MH  - Polymorphism, Single Nucleotide/*genetics/physiology
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Symporters/*genetics
MH  - K Cl- Cotransporters
EDAT- 2002/12/05 04:00
MHDA- 2003/06/11 05:00
CRDT- 2002/12/05 04:00
PHST- 2002/12/05 04:00 [pubmed]
PHST- 2003/06/11 05:00 [medline]
PHST- 2002/12/05 04:00 [entrez]
AID - 907 [pii]
AID - 10.1046/j.1365-2052.2002.00907.x [doi]
PST - ppublish
SO  - Anim Genet. 2002 Dec;33(6):455-9. doi: 10.1046/j.1365-2052.2002.00907.x.